Contactin 5 / CNTN5 Recombinant Protein | CNTN5 recombinant protein

Recombinant Human Contactin 5 / CNTN5 Protein (His tag)

Cat. Num. AAA2546212

• Gene Names: CNTN5; NB-2; HNB-2s
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Contactin 5 / CNTN5; Recombinant Human Contactin 5 / CNTN5 Protein (His tag); CNTN5; HNB-2s; MGC163491; NB-2; CNTN5 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1100

• Application Notes: The secreted recombinant human CNTN5 comprises 1052 amino acids with a predicted molecular mass of 116 kDa. As a result of glycosylation, rhCNTN5 migrates as an approximately 130-140 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Glu 19
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CNTN5 recombinant protein

Background: Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed mainly in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2 (TAG-1), Contactin-3 (BIG-1), BIG-2, Contactin-5 (NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-5, also known as NB-2, is one of the neural recognition molecules in the contactin subgroup. Contactin-5 is expressed in brain and kidney and at very low level in placenta. In brain, Contactin-5 is highly expressed in the occipital lobe, amygdala, cerebral cortex, frontal lobe, thalamus and temporal lobe. Mice deficient in the Contactin-5 gene exhibit aberrant responses to acoustic stimuli. Contactin-5 may play a role in maturation of glutamatergic synapses in the brainstem during the final stages of auditory development. Contactin-5 gene may contribute to human neurological disorders.

Description: A DNA sequence encoding the mature form of human CNTN5 (NP_055176.1) (Met 1-Gln 1059) with a C-terminal polyhistidine tag was expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 7657359
• NCBI GeneID: 53942
• NCBI Accession #: NP_055176.1
• NCBI GenBank Nucleotide #: NM_014361.3
• UniProt Accession #: O94779; O94780; Q49AF3; A1L4P0; B7ZM07; E9PKE8
• Molecular Weight: 100,327 Da
• NCBI Official Full Name: contactin-5 isoform 1
• NCBI Official Synonym Full Names: contactin 5
• NCBI Official Symbol: CNTN5
• NCBI Official Synonym Symbols: NB-2; HNB-2s
• NCBI Protein Information: contactin-5
• UniProt Protein Name: Contactin-5
• Protein Family: Contactin
• UniProt Gene Name: CNTN5
• UniProt Synonym Gene Names: hNB-2

NCBI Description

The protein encoded by this gene is a member of the immunoglobulin superfamily, and contactin family, which mediate cell surface interactions during nervous system development. This protein is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CNTN5: Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons. Probably involved in neuronal activity in the auditory system. Belongs to the immunoglobulin superfamily. Contactin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11q22.1

Cellular Component: cytoplasm