Immunohistochemistry (IHC)
(Immunochemical staining of mouse SERPINF2 in mouse liver with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
Rabbit anti-Mouse Alpha 2 Antiplasmin/SerpinF2 Monoclonal Antibody | anti-SerpinF2 antibody
Recombinant Anti-Alpha 2 Antiplasmin/SerpinF2 Antibody, Rabbit Monoclonal
Gene Names
Serpinf2; Pli; AI747498; Serpimf2
Reactivity
Mouse
Applications
ELISA, ELISA, Immunohistochemistry
Purity
Protein A
Synonyms
Alpha 2 Antiplasmin/SerpinF2; Monoclonal Antibody; Recombinant Anti-Alpha 2 Antiplasmin/SerpinF2 Antibody; Rabbit Monoclonal; SerpinF2/SERPINF2 Antibody; Rabbit MAb; serpin peptidase inhibitor; clade F; member 2; Anti-AI747498 Antibody; Anti-Pli Antibody; Anti-Serpimf2 Antibody; anti-SerpinF2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
128
Specificity
Mouse Serpin F2/SERPINF2.
No cross-reactivity in ELISA with Human Serpin A1/SERPINA1/AAT, Mouse Serpin D1/SERPIND1/HCF2
No cross-reactivity in ELISA with Human Serpin A1/SERPINA1/AAT, Mouse Serpin D1/SERPIND1/HCF2
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SerpinF2 antibody
ELISA (EIA), ELISA (EIA) (Cap), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:50-1:200
ELISA (Cap): 1:250-1:2000
ELISA (Cap): This antibody will detect Mouse SerpinF2 in ELISA pair set.
In a sandwich ELISA, it can be used as capture antibody.
IHC-P: 1:50-1:200
ELISA (Cap): 1:250-1:2000
ELISA (Cap): This antibody will detect Mouse SerpinF2 in ELISA pair set.
In a sandwich ELISA, it can be used as capture antibody.
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse SerpinF2 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse SerpinF2 (rM SerpinF2).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SERPINF2 in mouse liver with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SERPINF2 in mouse liver with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-SerpinF2 antibody
SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily. SerpinF2 is the principal physiological inhibitor of serine protease plasmin, and as well as, an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed in muscle, intestine, central nervous system, and placenta at a moderate level. It is indicated that Serpin F2 is a key regulator of plasmin-mediated proteolysis in these tissues. Alpha-2 AP is an unusual serpin in that it contains extensive N-and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. SerpinF2 is one of the inhibitors of fibrinolysis, which acts as the primary inhibitor of plasmin(ogen). It is a specific plasmin inhibitor, and is important in modulating the effectiveness and persistence of fibrin with respect to its susceptibility to digestion and removal by plasmin. Alpha-2 AP plays the dominant role in inhibiting both plasma clot lysis and thrombus lysis, and accordingly, the congenital deficiency of Alpha-2 antiplasmin causes a rare bleeding disorder because of increased fibrinolysis. Thus, it may be a useful target for developing more effective treatment of thrombotic diseases.
References
Lee KN, et al. (2004) Alpha2-antiplasmin: potential therapeutic roles in fibrin survival and removal. Curr Med Chem Cardiovasc Hematol Agents. 2(4): 303-10.Matsuno H. (2006) Alpha2-antiplasmin on cardiovascular diseases. Curr Pharm Des. 12(7): 841-7.Burnouf T, et al. (2007) Impact of Triton X-100 on alpha 2-antiplasmin (SERPINF2) activity in solvent/detergent-treated plasma. Biologicals. 35(4): 349-53.Carpenter SL, et al. (2008) Alpha2-antiplasmin and its deficiency: fibrinolysis out of balance. Haemophilia. 14(6): 1250-4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
160,000
NCBI Official Full Name
alpha-2-antiplasmin
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade F, member 2
NCBI Official Symbol
Serpinf2
NCBI Official Synonym Symbols
Pli; AI747498; Serpimf2
NCBI Protein Information
alpha-2-antiplasmin; serpin F2; alpha-2-AP; alpha-2-PI; alpha 2 antiplasmin; alpha-2-plasmin inhibitor; plasmin inhibitor alpha 2; pigment epithelium derived factor; serine (or cysteine) proteinase inhibitor, clade F, member 2
UniProt Protein Name
Alpha-2-antiplasmin
Protein Family
UniProt Gene Name
Serpinf2
UniProt Synonym Gene Names
Pli; Alpha-2-AP; Alpha-2-PI
UniProt Entry Name
A2AP_MOUSE
Uniprot Description
SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; protein homodimerization activity; protease binding; protease inhibitor activity
Biological Process: negative regulation of fibrinolysis; collagen fibril organization; renin-angiotensin regulation of blood vessel size; positive regulation of smooth muscle cell proliferation; positive regulation of stress fiber formation; positive regulation of collagen biosynthetic process; acute-phase response; positive regulation of JNK cascade; negative regulation of peptidase activity; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation
Disease: Alpha-2-plasmin Inhibitor Deficiency
Research Articles on SerpinF2
Similar Products
Product Notes
The SerpinF2 serpinf2 (Catalog #AAA8111130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Alpha 2 Antiplasmin/SerpinF2 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha 2 Antiplasmin/SerpinF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), ELISA (EIA) (Cap), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:50-1:200 ELISA (Cap): 1:250-1:2000 ELISA (Cap): This antibody will detect Mouse SerpinF2 in ELISA pair set. In a sandwich ELISA, it can be used as capture antibody. Researchers should empirically determine the suitability of the SerpinF2 serpinf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha 2 Antiplasmin/SerpinF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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