Rabbit anti-Human Alpha 2 Antiplasmin/SerpinF2 Polyclonal Antibody | anti-SerpinF2 antibody

Anti-Alpha 2 Antiplasmin/SerpinF2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102164

• Gene Names: SERPINF2; AAP; API; PLI; A2AP; ALPHA-2-PI
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: Alpha 2 Antiplasmin/SerpinF2; Polyclonal Antibody; Anti-Alpha 2 Antiplasmin/SerpinF2 Antibody; Rabbit Polyclonal; SerpinF2/AAP Antibody; Rabbit PAb; serpin peptidase inhibitor; clade F; member 2; Anti-A2AP Antibody; Anti-AAP Antibody; Anti-ALPHA-2-PI Antibody; Anti-API Antibody; Anti-PLI Antibody; anti-SerpinF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Alpha 2 Antiplasmin/SerpinF2
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SerpinF2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:1000-1:2000
• Immunogen: Recombinant Human SerpinF2 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human SerpinF2 (rh SerpinF2). Total IgG was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SerpinF2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa)

Related Product Information for anti-SerpinF2 antibody

SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily. SerpinF2 is the principal physiological inhibitor of serine protease plasmin, and as well as, an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed in muscle, intestine, central nervous system, and placenta at a moderate level. It is indicated that Serpin F2 is a key regulator of plasmin-mediated proteolysis in these tissues. Alpha-2 AP is an unusual serpin in that it contains extensive N-and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. SerpinF2 is one of the inhibitors of fibrinolysis, which acts as the primary inhibitor of plasmin(ogen). It is a specific plasmin inhibitor, and is important in modulating the effectiveness and persistence of fibrin with respect to its susceptibility to digestion and removal by plasmin. Alpha-2 AP plays the dominant role in inhibiting both plasma clot lysis and thrombus lysis, and accordingly, the congenital deficiency of Alpha-2 antiplasmin causes a rare bleeding disorder because of increased fibrinolysis. Thus, it may be a useful target for developing more effective treatment of thrombotic diseases.

References

Lee KN, et al. (2004) Alpha2-antiplasmin: potential therapeutic roles in fibrin survival and removal. Curr Med Chem Cardiovasc Hematol Agents. 2(4): 303-10.Matsuno H. (2006) Alpha2-antiplasmin on cardiovascular diseases. Curr Pharm Des. 12(7): 841-7.Burnouf T, et al. (2007) Impact of Triton X-100 on alpha 2-antiplasmin (SERPINF2) activity in solvent/detergent-treated plasma. Biologicals. 35(4): 349-53.Carpenter SL, et al. (2008) Alpha2-antiplasmin and its deficiency: fibrinolysis out of balance. Haemophilia. 14(6): 1250-4.

NCBI and Uniprot Product Information

• NCBI GI #: 115583663
• NCBI GeneID: 5345
• NCBI Accession #: NP_000925.2
• NCBI GenBank Nucleotide #: NM_000934.3
• UniProt Accession #: P08697; Q8N5U7; Q9UCG2; Q9UCG3; B4E1B7
• Molecular Weight: 54,566 Da
• NCBI Official Full Name: alpha-2-antiplasmin isoform a
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
• NCBI Official Symbol: SERPINF2
• NCBI Official Synonym Symbols: AAP; API; PLI; A2AP; ALPHA-2-PI
• NCBI Protein Information: alpha-2-antiplasmin; serpin F2; alpha-2-AP; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
• UniProt Protein Name: Alpha-2-antiplasmin
• Protein Family: Alpha-2-antiplasmin
• UniProt Gene Name: SERPINF2
• UniProt Synonym Gene Names: AAP; PLI; Alpha-2-AP; Alpha-2-PI
• UniProt Entry Name: A2AP_HUMAN

NCBI Description

This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; protease binding; endopeptidase inhibitor activity

Biological Process: platelet activation; collagen fibril organization; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; response to organic substance; fibrinolysis; platelet degranulation; negative regulation of fibrinolysis; renin-angiotensin regulation of blood vessel size; positive regulation of stress fiber formation; acute-phase response; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; blood coagulation

Disease: Alpha-2-plasmin Inhibitor Deficiency