Typical Testing Data/Standard Curve (for reference only)
RBP4 elisa kit
RBP4 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to rat TNF-alpha. Samples are pipetted into these wells. Nonbound rat TNF-alpha and other components of the sample should be removed by washing, then monoclonal antibody specific to rat TNF-alpha added. In order to quantitatively determine the amount of rat TNF-alpha present in the sample, Streptavidin Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured rat TNF-alpha!!Background/Introduction: Tumor necrosis factor (TNF)-alpha is an important cytokine in the innate immune response produced by macrophages, neutrophils, fibroblasts, keratinocytes, NK cells, T and B cells, and tumour cells. TNF-alpha mediates host responses in acute and chronic inflammatory conditions, and is a mediator of protection from infection and malignancy. TNF-alpha causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis, and viral replication. TNF-alpha is initially produced as a biologically active 26 kDa transmembrane protein, which is subsequently cleaved, principally by TNF-alpha-converting enzyme (TACE), to release the 17kDa free protein. These proteins are arranged in biologically active homotrimers that act on the ubiquitously expressed TNF-alpha receptors 1 and 2 (TNFR1 and TNFR2). These 17 kDa TNF protomers are composed of two antiparallel beta-pleated sheets with antiparallel beta-strands, forming a 'jelly roll' beta-structure, typical for the TNF family. TNF-R1 is constitutively expressed in most tissues, and can be fully activated by both the membrane-bound and soluble trimeric forms of TNF, while TNF-R2 is only found in cells of the immune system and respond to the membranebound form of the TNF homotrimer. Although their extracellular domains share structural and functional homology, their intracellular domains are distinct. Tumor necrosis factor promotes the inflammatory response, which in turn causes many of the clinical problems associated with autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, Crohn's disease, psoriasis and refractory asthma. These disorders are sometimes treated by using TNF inhibitors such as infliximab (Remicade) or adalimumab (Humira).
NCBI and Uniprot Product Information
NCBI Description
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]
Uniprot Description
RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 10q23.33
Cellular Component: cytosol; extracellular region; extracellular space; protein complex
Molecular Function: protein binding; protein heterodimerization activity; retinal binding; retinol binding
Biological Process: cardiac muscle development; embryonic organ morphogenesis; embryonic retina morphogenesis in camera-type eye; embryonic skeletal development; eye development; fat-soluble vitamin metabolic process; female genitalia morphogenesis; gluconeogenesis; glucose homeostasis; heart development; lung development; maintenance of gastrointestinal epithelium; negative regulation of cardiac muscle cell proliferation; phototransduction, visible light; positive regulation of immunoglobulin secretion; positive regulation of insulin secretion; response to ethanol; response to retinoic acid; retinoid metabolic process; retinol metabolic process; urinary bladder development; uterus development; vagina development; visual perception; vitamin metabolic process
Disease: Microphthalmia, Isolated, With Coloboma 10; Retinal Dystrophy, Iris Coloboma, And Comedogenic Acne Syndrome
Research Articles on RBP4
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Product Notes
The RBP4 rbp4 (Catalog #AAA495208) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RBP4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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