Rat Retinol-binding protein 4 ELISA Kit | Rbp4 elisa kit

Rat Retinol-binding protein 4 ELISA Kit

Cat. Num. AAA764124

• Gene Names: RBP4; RDCCAS; MCOPCB10
• Reactivity: Rat
• Synonyms: Retinol-binding protein 4; Rat Retinol-binding protein 4 ELISA Kit; Rbp4/RBP4 (Retinol-Binding Protein 4)/interstitial/Plasma retinol-binding protein/retinol binding protein 4; plasma/retinol-binding protein 4; plasma; Rbp4 elisa kit

• Reactivity: Rat
• Sequence Length: 201

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Rbp4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Rbp4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Rbp4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Rbp4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Rbp4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 55743122
• NCBI GeneID: 5950
• NCBI Accession #: NP_006735.2
• NCBI GenBank Nucleotide #: NM_006744.3
• Molecular Weight: 23,010 Da
• NCBI Official Full Name: retinol-binding protein 4
• NCBI Official Synonym Full Names: retinol binding protein 4
• NCBI Official Symbol: RBP4
• NCBI Official Synonym Symbols: RDCCAS; MCOPCB10
• NCBI Protein Information: retinol-binding protein 4
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: RBP4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_HUMAN

NCBI Description

This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]

Uniprot Description

RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: cytosol; extracellular region; extracellular space; protein complex

Molecular Function: protein binding; protein heterodimerization activity; retinal binding; retinol binding

Biological Process: cardiac muscle development; embryonic organ morphogenesis; embryonic retina morphogenesis in camera-type eye; embryonic skeletal development; eye development; fat-soluble vitamin metabolic process; female genitalia morphogenesis; gluconeogenesis; glucose homeostasis; heart development; lung development; maintenance of gastrointestinal epithelium; negative regulation of cardiac muscle cell proliferation; phototransduction, visible light; positive regulation of immunoglobulin secretion; positive regulation of insulin secretion; response to ethanol; response to retinoic acid; retinoid metabolic process; retinol metabolic process; urinary bladder development; uterus development; vagina development; visual perception; vitamin metabolic process

Disease: Microphthalmia, Isolated, With Coloboma 10; Retinal Dystrophy, Iris Coloboma, And Comedogenic Acne Syndrome