CD98 Recombinant Protein | SLC3A2 recombinant protein

CD98 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122999

• Gene Names: Slc3a2; 4F2; Cd98; Ly10; Mdu1; 4F2HC; Ly-10; NACAE; Ly-m10; Mgp-2hc; AI314110
• Purity: >98% as determined by SDS-PAGE
• Synonyms: CD98; CD98 Protein; Mouse; Recombinant (His Tag); Mouse SLC3A2/CD98 Protein (His Tag); 4F2 Protein; 4F2HC Protein; AI314110 Protein; Cd98 Protein; Ly-10 Protein; Ly-m10 Protein; Ly10 Protein; Mdu1 Protein; Mgp-2hc Protein; NACAE Protein; solute carrier family 3 (amino acid transporter heavy chain); member 2; SLC3A2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala139-Ala565

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse SLC3A2 (NP_001154885.1)(Ala 139-Ala 565) was fused with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SLC3A2 recombinant protein

Background: 4F2 cell-surface antigen heavy chain, also known as 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, CD98, SLC3A2 and MDU1, is a single-pass type I I membrane protein that belongs to the SLC3A transporter family. SLC3A2/MDU1 is expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta is significantly stronger at full-term than at the mid-trimester stage. SLC3A2/MDU1 is expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. It is expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. SLC3A2/MDU1 is also expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco2-BBE. SLC3A2/MDU1 is required for the function of light chain amino-acid transporters. It is involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. SLC3A2/MDU1 is involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, SLC3A2/MDU1 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. SLC3A2/MDU1 plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. It is required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, SLC3A2/MDU1 is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta.

References

Torrents D. et al., 1998, J Biol Chem. 273: 32437-45. Pfeiffer R. et al., 1999, EMBO J. 18: 49-57. Broeer A. et al., 2000, Biochem J. 349: 787-95. Yanagida O. et al., 2001, Biochim Biophys Acta. 1514: 291-302. Broeer A. et al., 2001, Biochem J. 355: 725-31. Fort J. et al., 2007, J Biol Chem. 282: 31444-52. Mayya V. et al., 2009, Sci Signal. 2: RA46-RA46.

NCBI and Uniprot Product Information

• NCBI GI #: 238637277
• NCBI GeneID: 17254
• NCBI Accession #: NP_001154885.1
• NCBI GenBank Nucleotide #: NM_001161413.1
• UniProt Accession #: P10852; Q54AH5; G3UWA6
• Molecular Weight: 62,240 Da
• NCBI Official Full Name: 4F2 cell-surface antigen heavy chain isoform a
• NCBI Official Synonym Full Names: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• NCBI Official Symbol: Slc3a2
• NCBI Official Synonym Symbols: 4F2; Cd98; Ly10; Mdu1; 4F2HC; Ly-10; NACAE; Ly-m10; Mgp-2hc; AI314110
• NCBI Protein Information: 4F2 cell-surface antigen heavy chain; CD98 antigen; CD98 heavy chain; antigen identified by monoclonal antibodies 4F2; solute carrier family 3 member 2; type II transmembrane protein
• UniProt Protein Name: 4F2 cell-surface antigen heavy chain
• Protein Family: 4F2 cell-surface antigen
• UniProt Gene Name: Slc3a2
• UniProt Synonym Gene Names: Mdu1; 4F2hc
• UniProt Entry Name: 4F2_MOUSE

Uniprot Description

SLC3A2: a transmembrane solute carrier protein that transports L-type amino acids and regulates intracellular calcium levels. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. Disulfide-linked heterodimer of a glycosylated heavy chain and a non-glycosylated light chain (SLC7A5, SLC7A6, SLCA7A7, SLC7A8, SLC7A10 or SLCA7A11). Colocalizes with cadherins. Interacts with FAM57A and ICAM1. Constitutively and specifically associates with beta-1 integrins (alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1 and alpha-6/beta-1), but minimally with alpha-4/beta-1. Expression is induced in resting peripheral blood T- lymphocytes following PHA stimulation. Expression increases at the time of maximal DNA synthesi, in fibroblasts stimulated to divide. Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid- trimester stage. Belongs to the SLC3A transporter family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, integral

Cellular Component: nucleoplasm; cell surface; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus

Molecular Function: cation binding; protein binding; neutral amino acid transmembrane transporter activity; double-stranded RNA binding; catalytic activity

Biological Process: neutral amino acid transport; response to exogenous dsRNA; amino acid transport; transport; carbohydrate metabolic process