Typical Testing Data/Standard Curve (for reference only)
Resistin ELISA Kit | RETN elisa kit
Resistin (human) Serum ELISA Kit
Gene Names
RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1
Synonyms
Resistin; Resistin (human) Serum ELISA Kit; RETN elisa kit
Sequence Length
108
Samples
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
Assay Type
Sandwich
Features & Benefits
Simple procedure
Fast and convenient
High-throughput adaptable
Fast and convenient
High-throughput adaptable
Preparation and Storage
4 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RETN elisa kit
Product Information: Resistin (human) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human Resistin in Serum, Plasma or Cell Culture Supernatant. Assay Range 0.125-8 ng/ml and Detection limit 100 pg/ml.100 Assays.
Background: Obesity is a well-known risk factor of type 2 diabetes mellitus and is strongly associated with insulin resistance. Resistin (also called FIZZ3/ADSF) is an adipocyte-derived peptide first identified during a search for targets of thiazolidinediones. Serum concentrations of resistin are markedly increased in obese mice and are decreased by treatment with thiazolidinediones. It was also found that administration of an anti-resistin antibody increases insulin-stimulated glucose uptake in obese mice and that treatment of normal mice with recombinant resistin impairs insulin action. Thus, resistin might link obesity with insulin resistance and diabetes in mice models. However, subsequent studies in rodent models have produced disparate findings on the role of resistin in obesity and insulin resistance. In humans, while the expression of resistin in human adipocytes is very low compared with that seen in rodents and does not differ between normal, insulin-resistant or type 2 diabetic individuals, a more recent study using a large size of case suggests that the plasma resistin levels are increased in type 2 diabetes. Therefore determination of the plasma resistin levels may be important for understanding onsets of metabolic diseases such as type 2 diabetes or obesity. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human resistin in biological fluids. A monoclonal antibody specific for resistin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, resistin is recognized by the addition of a biotinylated polyclonal antibody specific for resistin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of resistin in the samples. The assay range is 0.125-8 ng/ml resistin/ml. The lowest level of resistin that can be detected by this assay is 100 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications- This assay detects human Resistin in the range of 0.125-8 ng/ml resistin/ml. The lowest level of resistin that can be detected by this assay is 100 pg/ml.
Background: Obesity is a well-known risk factor of type 2 diabetes mellitus and is strongly associated with insulin resistance. Resistin (also called FIZZ3/ADSF) is an adipocyte-derived peptide first identified during a search for targets of thiazolidinediones. Serum concentrations of resistin are markedly increased in obese mice and are decreased by treatment with thiazolidinediones. It was also found that administration of an anti-resistin antibody increases insulin-stimulated glucose uptake in obese mice and that treatment of normal mice with recombinant resistin impairs insulin action. Thus, resistin might link obesity with insulin resistance and diabetes in mice models. However, subsequent studies in rodent models have produced disparate findings on the role of resistin in obesity and insulin resistance. In humans, while the expression of resistin in human adipocytes is very low compared with that seen in rodents and does not differ between normal, insulin-resistant or type 2 diabetic individuals, a more recent study using a large size of case suggests that the plasma resistin levels are increased in type 2 diabetes. Therefore determination of the plasma resistin levels may be important for understanding onsets of metabolic diseases such as type 2 diabetes or obesity. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human resistin in biological fluids. A monoclonal antibody specific for resistin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, resistin is recognized by the addition of a biotinylated polyclonal antibody specific for resistin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of resistin in the samples. The assay range is 0.125-8 ng/ml resistin/ml. The lowest level of resistin that can be detected by this assay is 100 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications- This assay detects human Resistin in the range of 0.125-8 ng/ml resistin/ml. The lowest level of resistin that can be detected by this assay is 100 pg/ml.
Product Categories/Family for RETN elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
8,686 Da
NCBI Official Full Name
resistin
NCBI Official Synonym Full Names
resistin
NCBI Official Symbol
RETN
NCBI Official Synonym Symbols
ADSF; RSTN; XCP1; FIZZ3; RETN1
NCBI Protein Information
resistin
UniProt Protein Name
Resistin
Protein Family
UniProt Gene Name
RETN
UniProt Synonym Gene Names
FIZZ3; HXCP1; RSTN; ADSF
UniProt Entry Name
RETN_HUMAN
NCBI Description
This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]
Uniprot Description
resistin: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Belongs to the resistin/FIZZ family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: extracellular space; nucleus
Molecular Function: hormone activity
Biological Process: fat cell differentiation; response to mechanical stimulus; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; positive regulation of synaptic transmission; aging; response to insulin stimulus
Disease: Diabetes Mellitus, Noninsulin-dependent
Research Articles on RETN
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Product Notes
The RETN retn (Catalog #AAA843352) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Resistin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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