DNA Damage (8-OHdG) ELISA Kit | 8-OHdG elisa kit
DNA Damage (8-OHdG) ELISA Kit
Synonyms
DNA Damage (8-OHdG); DNA Damage (8-OHdG) ELISA Kit; DNA Damage ELISA Kit; 8-hydroxy-2-deoxyguanosine (8-OHdG); 8-OHdG elisa kit
Application Notes
1. Prepare the standard and samples in the Sample and Standard Diluent.
2. Add 50 uL of prepared standards and samples in triplicate to appropriate wells.
3. Add 50 uL of the diluted antibody preparation to the appropriate wells.
4. Cover plate with Plate Cover and incubate at room temperature (20-25 degree C) for 1 hour.
5. Dilute 10X Wash Buffer 1:10 with diH2O.
6. Wash plate 4 times with 1X Wash Buffer.
7. Add 100 uL TMB Substrate to each well.
8. Cover plate with Plate Cover and incubate at room temperature in the dark for 30 minutes to develop the signal.
9. Add 100 uL Stop Solution to each well.
10. Measure absorbance using a plate reader at 450 nm.
11. Plot the standard curve and calculate sample concentrations.
2. Add 50 uL of prepared standards and samples in triplicate to appropriate wells.
3. Add 50 uL of the diluted antibody preparation to the appropriate wells.
4. Cover plate with Plate Cover and incubate at room temperature (20-25 degree C) for 1 hour.
5. Dilute 10X Wash Buffer 1:10 with diH2O.
6. Wash plate 4 times with 1X Wash Buffer.
7. Add 100 uL TMB Substrate to each well.
8. Cover plate with Plate Cover and incubate at room temperature in the dark for 30 minutes to develop the signal.
9. Add 100 uL Stop Solution to each well.
10. Measure absorbance using a plate reader at 450 nm.
11. Plot the standard curve and calculate sample concentrations.
Sample
Culture medium, urine, plasma/serum, cell lysates, tissue samples, saliva
Excitation/Emission
Absorbance Plate Reader
Preparation and Storage
Store 8-OHdG at -20 degree C. All other kit components at 2-8 degree C.
Related Product Information for 8-OHdG elisa kit
Description: Quantify 8-OHdG in urine, cell culture, plasma and other sample matrices using DNA Damage (8-OHdG) ELISA Kit. This kit offers a quick incubation time, stable reagents, and a user-friendly protocol. Analyze results using an absorbance plate reader.
Background: 8-hydroxy-2-deoxyguanosine (8-OHdG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension. Quantify 8-OHdG in urine, cell culture, plasma and other sample matrices using DNA Damage ELISA Kit. This kit offers a quick incubation time, stable reagents, and a user-friendly protocol.
The ELISA utilizes an 8-hydroxy-2-deoxyguanosine-coated plate and an HRP-conjugated 8-OHdG specific detection antibody. This produces an assay with a useful range of 0.94-60 ng/mL, and a sensitivity of 0.59 ng/mL. Analyze your results using an absorbance plate reader.
Background: 8-hydroxy-2-deoxyguanosine (8-OHdG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension. Quantify 8-OHdG in urine, cell culture, plasma and other sample matrices using DNA Damage ELISA Kit. This kit offers a quick incubation time, stable reagents, and a user-friendly protocol.
The ELISA utilizes an 8-hydroxy-2-deoxyguanosine-coated plate and an HRP-conjugated 8-OHdG specific detection antibody. This produces an assay with a useful range of 0.94-60 ng/mL, and a sensitivity of 0.59 ng/mL. Analyze your results using an absorbance plate reader.
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Product Notes
The 8-OHdG (Catalog #AAA258375) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 1. Prepare the standard and samples in the Sample and Standard Diluent. 2. Add 50 uL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 uL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25 degree C) for 1 hour. 5. Dilute 10X Wash Buffer 1:10 with diH2O. 6. Wash plate 4 times with 1X Wash Buffer. 7. Add 100 uL TMB Substrate to each well. 8. Cover plate with Plate Cover and incubate at room temperature in the dark for 30 minutes to develop the signal. 9. Add 100 uL Stop Solution to each well. 10. Measure absorbance using a plate reader at 450 nm. 11. Plot the standard curve and calculate sample concentrations. Researchers should empirically determine the suitability of the 8-OHdG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Damage (8-OHdG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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