Western Blot (WB)
(Western blot analysis of Alpha A Crystallin expression in rat spleen extract (lane 1), mouse eye ball extract (lane 2) and SMMC7721 whole cell lysates (lane 3). Alpha A Crystallin at 20KD was detected using rabbit anti- Alpha A Crystallin Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit Crystallin Polyclonal Antibody | anti-CRYAA antibody
Anti-Alpha A Crystallin Antibody
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Mouse, Rat; Predicted Species: Human
Tested Species:In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of Alpha A Crystallin expression in rat spleen extract (lane 1), mouse eye ball extract (lane 2) and SMMC7721 whole cell lysates (lane 3). Alpha A Crystallin at 20KD was detected using rabbit anti- Alpha A Crystallin Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of Alpha A Crystallin expression in rat spleen extract (lane 1), mouse eye ball extract (lane 2) and SMMC7721 whole cell lysates (lane 3). Alpha A Crystallin at 20KD was detected using rabbit anti- Alpha A Crystallin Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Immunohistochemistry (IHC)
(Alpha A Crystallin was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti- Alpha A Crystallin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(Alpha A Crystallin was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti- Alpha A Crystallin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(Alpha A Crystallin was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- Alpha A Crystallin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(Alpha A Crystallin was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- Alpha A Crystallin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )
Background: Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).
2. Derham BK, Harding JJ (1999). "Alpha-crystallin as a molecular chaperone.". Progress in retinal and eye research 18 (4): 463-509.
3. Jaworski CJ, Piatigorsky J (1989). "A pseudo-exon in the functional human alpha A-crystallin gene.". Nature337 (6209): 752-4.
NCBI and Uniprot Product Information
NCBI Description
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]
Uniprot Description
CRYA1: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP, also known as the HSP20) family. Alpha-A is preferentially restricted to the lens.
Protein type: Chaperone; Heat shock protein
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: cytoplasm; nucleus
Molecular Function: identical protein binding; protein binding; unfolded protein binding
Biological Process: negative regulation of apoptosis; negative regulation of intracellular transport; protein homooligomerization; protein refolding; visual perception
Disease: Cataract 9, Multiple Types
Research Articles on CRYAA
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Product Notes
The CRYAA cryaa (Catalog #AAA178440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alpha A Crystallin Antibody reacts with Human, Mouse, Rat No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Crystallin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Mouse, Rat Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Mouse, Rat; Predicted Species: Human Tested Species:In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Researchers should empirically determine the suitability of the CRYAA cryaa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Crystallin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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