Rabbit anti-Human MRC2 Polyclonal Antibody | anti-MRC2 antibody

MRC2, ID (MRC2, CLEC13E, ENDO180, KIAA0709, UPARAP, C-type mannose receptor 2, C-type lectin domain family 13 member E, Endocytic receptor 180, Macrophage mannose receptor 2, Urokinase-type plasminogen activator receptor-associated protein, CD280) (Biotin

Cat. Num. AAA6322056

• Gene Names: MRC2; CD280; UPARAP; CLEC13E; ENDO180
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MRC2; Polyclonal Antibody; ID (MRC2; CLEC13E; ENDO180; KIAA0709; UPARAP; C-type mannose receptor 2; C-type lectin domain family 13 member E; Endocytic receptor 180; Macrophage mannose receptor 2; Urokinase-type plasminogen activator receptor-associated protein; CD280) (Biotin; CD280; anti-MRC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MRC2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MRC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 797-827 amino acids from the Central region of human MRC2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MRC2 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MRC2 antibody detected the MRC2 protein (arrow).)

Related Product Information for anti-MRC2 antibody

MRC2 is a recycling endocytic receptor that functions in cell motility and remodeling of the extracellular matrix by promoting cell migration and uptake of collagens for intracellular degradation (Wienke et al., 2007 [PubMed 17974964]).[supplied by OMIM].

Product Categories/Family for anti-MRC2 antibody

Antibodies; CD Markers

References

Messaritou, G., et al. J. Cell. Sci. 122 (PT 22), 4042-4048 (2009): Wu, X., et al. Cancer Prev Res (Phila Pa) 2(7):617-624(2009). Wienke, D., et al. Cancer Res. 67(21):10230-10240(2007). Sulek, J., et al. J. Histochem. Cytochem. 55(4):347-353(2007). Johnson, J.M., et al. Science 302(5653):2141-2144(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 110624774
• NCBI GeneID: 9902
• NCBI Accession #: NP_006030.2
• NCBI GenBank Nucleotide #: NM_006039.4
• UniProt Accession #: Q9UBG0; Q7LGE7; Q9Y5P9; A6H8K4; D3DU08
• Molecular Weight: 166,674 Da
• NCBI Official Full Name: C-type mannose receptor 2
• NCBI Official Synonym Full Names: mannose receptor, C type 2
• NCBI Official Symbol: MRC2
• NCBI Official Synonym Symbols: CD280; UPARAP; CLEC13E; ENDO180
• NCBI Protein Information: C-type mannose receptor 2; endocytic receptor 180; UPAR-associated protein; macrophage mannose receptor 2; C-type lectin domain family 13 member E; endocytic receptor (macrophage mannose receptor family); urokinase-type plasminogen activator receptor-asso
• UniProt Protein Name: C-type mannose receptor 2
• Protein Family: C-type mannose receptor
• UniProt Gene Name: MRC2
• UniProt Synonym Gene Names: CLEC13E; ENDO180; KIAA0709; UPARAP; UPAR-associated protein
• UniProt Entry Name: MRC2_HUMAN

NCBI Description

This gene encodes a member of the mannose receptor family of proteins that contain a fibronectin type II domain and multiple C-type lectin-like domains. The encoded protein plays a role in extracellular matrix remodeling by mediating the internalization and lysosomal degradation of collagen ligands. Expression of this gene may play a role in the tumorigenesis and metastasis of several malignancies including breast cancer, gliomas and metastatic bone disease. [provided by RefSeq, Feb 2012]

Uniprot Description

MRC2: May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs).

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: focal adhesion; membrane; integral to membrane

Molecular Function: collagen binding; protein binding; carbohydrate binding

Biological Process: osteoblast differentiation; collagen catabolic process; endocytosis