Immunohistochemistry (IHC)
((0. 05ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med. , Budapest, Hungary.)
Goat OXT Polyclonal Antibody | anti-OXT antibody
Goat anti-OXT Antibody
Immunohistochemistry: Frozen section of Human Hypothalamus shows staining of magnocellular oxytocin neurons in the supraoptic nucleus. Recommended concentration, 0.05-0.15ug/ml.
Western Blot: Preliminary experiments in Human Brain (Cerebellum, Amygdala) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Immunohistochemistry (IHC)
((0. 05ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med. , Budapest, Hungary.)
Immunohistochemistry (IHC)
((0. 05ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med. , Budapest, Hungary.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a precursor protein that is processed to produce oxytocin and neurophysin I. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. [provided by RefSeq, Dec 2013]
Uniprot Description
OXT: Neurophysin 1 specifically binds oxytocin. Belongs to the vasopressin/oxytocin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 20p13
Cellular Component: cytosol; extracellular region; extracellular space; secretory granule; terminal button
Molecular Function: neurohypophyseal hormone activity; oxytocin receptor binding
Biological Process: drinking behavior; eating behavior; elevation of cytosolic calcium ion concentration; female pregnancy; grooming behavior; heart development; hyperosmotic salinity response; male mating behavior; maternal behavior; memory; negative regulation of blood pressure; positive regulation of blood pressure; positive regulation of female receptivity; positive regulation of ossification; positive regulation of prostaglandin secretion; positive regulation of synaptic transmission; positive regulation of synaptogenesis; regulation of heart rate; regulation of sensory perception of pain; response to activity; response to amphetamine; response to cAMP; response to cocaine; response to electrical stimulus; response to estradiol stimulus; response to ether; response to food; response to glucocorticoid stimulus; response to peptide hormone stimulus; response to progesterone stimulus; response to retinoic acid; response to sucrose stimulus; signal transduction; sleep; social behavior; sperm ejaculation
Research Articles on OXT
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Product Notes
The OXT oxt (Catalog #AAA421673) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-OXT Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's OXT can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: Frozen section of Human Hypothalamus shows staining of magnocellular oxytocin neurons in the supraoptic nucleus. Recommended concentration, 0.05-0.15ug/ml. Western Blot: Preliminary experiments in Human Brain (Cerebellum, Amygdala) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). Researchers should empirically determine the suitability of the OXT oxt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HADPACDAEA TFSQR. It is sometimes possible for the material contained within the vial of "OXT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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