Typical Testing Data/Standard Curve (for reference only)
CXCL1 / GROalpha elisa kit
CXCL1 / GROalpha (Mouse) ELISA Kit
Synonyms
CXCL1 / GROalpha; CXCL1 / GROalpha (Mouse) ELISA Kit; Chemokine (C-X-C motif) ligand 1; CXCL1; GRO1 oncogene; GROalpha; KC; Neutrophil-activating protein 3; NAP-3; Melanoma growth stimulating activity; alpha; MSGA-alpha; CXCL1 / GROalpha elisa kit
Samples
Serum, Plasma, Body Fluids, Tissue Lysates Or Cell Culture Supernates
Assay Type
Sandwich
Detection Range
31.2 pg/ml-2000 pg/ml
Sensitivity
< 2 pg/ml
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CXCL1 / GROalpha elisa kit
Intended Uses: The mouse BDNF ELISA has been designed for the precise analytical measurement of BDNF concentrations in serum, plasma, body fluids, tissue lysates or cell culture supernates from mice. For research use only.
Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. Anti-BDNF polyclonal antibody was pre-coated onto 96-well plates. The biotin conjugated anti-BDNF polyclonal second antibody was used as detection antibody. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The optical density of the yellow color is proportional to the BDNF amount of the sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BDNF in the sample can be calculated!!Background/Introduction: Brain-derived neurotrophic factor, also known as BDNF, is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. BDNF is a member of the "neurotrophin" family of growth factors, which are related to the NGF. It acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain—areas vital to learning, memory, and higher thinking. Post mortem analysis has shown lowered levels of BDNF in the brain tissues of people with Alzheimer's disease.
Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. Anti-BDNF polyclonal antibody was pre-coated onto 96-well plates. The biotin conjugated anti-BDNF polyclonal second antibody was used as detection antibody. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The optical density of the yellow color is proportional to the BDNF amount of the sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BDNF in the sample can be calculated!!Background/Introduction: Brain-derived neurotrophic factor, also known as BDNF, is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. BDNF is a member of the "neurotrophin" family of growth factors, which are related to the NGF. It acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain—areas vital to learning, memory, and higher thinking. Post mortem analysis has shown lowered levels of BDNF in the brain tissues of people with Alzheimer's disease.
Product Categories/Family for CXCL1 / GROalpha elisa kit
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Product Notes
The CXCL1 / GROalpha (Catalog #AAA495352) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CXCL1 / GROalpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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