Cetuximab ELISA Kit
Cetuximab Antibody ELISA Kit
Synonyms
Cetuximab; Cetuximab Antibody ELISA Kit; Cetuximab elisa kit
Specificity
Based on the approach used in calculating the assay cut-off value of anti-drug antibody (ADA) ELISAs, approximately 1-5% of tested samples are expected to generate false-positive ADA response during initial screening analysis. Therefore, further confirmation of the specificity of the ADA activity in the samples, identified as positive, is highly recommended. This is supported also by recent publications. In order to confirm the specificity of the ADA signal, a confirmatory assay (competitive drug inhibition test) is proposed by ImmunoGuide. The confirmatory assay is performed with the Confirmatory Reagent (coded IG-CR112). This Confirmatory Reagent is available as a separate product and can be obtained from ImmunoGuide upon request.
Samples
Serum, Urine And Platelets
Assay Type
Quantitative Competitive
Preparation and Storage
2 - 8 degree C
Related Product Information for Cetuximab elisa kit
Intended Uses: Enzyme Immunoassay for the quantitative determination of Serotonin in serum, urine and platelets.
Principle of the Assay: In the first step, Serotonin is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.
Principle of the Assay: In the first step, Serotonin is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.
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Product Notes
The Cetuximab (Catalog #AAA495348) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cetuximab, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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