Typical Testing Data/Standard Curve (for reference only)
Human Tryptophan ELISA Kit
Tryptophan ELISA, 96 well
Reactivity
Human
Synonyms
Tryptophan; Tryptophan ELISA; 96 well; Tryptophan elisa kit
Reactivity
Human
Samples
Urine, serum and plasma samples
Assay Type
Quantitative Competitive
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Tryptophan elisa kit
Intended Uses: Enzyme Immunoassay for the quantitative determination of Tryptophan in urine, serum and plasma samples.
Principle of the Assay: After extraction and derivatization Tryptophan is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standards.
Background/Introduction: L-Tryptophan is one of the essential amino acids for the human metabolism and must be part of its diet. In humans it serves as precursor for the synthesis of the neurotransmitters serotonin and tryptamine as well as for the synthesis of nicotinic acid and the epiphyseal hormone melatonin. Tryptophan is catabolized to kynurenine through the enzyme IDO (indoleamine-2,3-dioxygenase). Increased IDO activity is an expression of neuro-endocrine-immunological dysregulation, which is often associated with depressive symptoms such as bipolar disorder (manic depression). In addition, Tryptophan and its metabolites regulate neurobehavioral effects such as appetite, sleeping-waking-rhythm and pain perception.
Principle of the Assay: After extraction and derivatization Tryptophan is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standards.
Background/Introduction: L-Tryptophan is one of the essential amino acids for the human metabolism and must be part of its diet. In humans it serves as precursor for the synthesis of the neurotransmitters serotonin and tryptamine as well as for the synthesis of nicotinic acid and the epiphyseal hormone melatonin. Tryptophan is catabolized to kynurenine through the enzyme IDO (indoleamine-2,3-dioxygenase). Increased IDO activity is an expression of neuro-endocrine-immunological dysregulation, which is often associated with depressive symptoms such as bipolar disorder (manic depression). In addition, Tryptophan and its metabolites regulate neurobehavioral effects such as appetite, sleeping-waking-rhythm and pain perception.
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Product Notes
The Human Tryptophan (Catalog #AAA495079) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495079 ELISA Kit recognizes Human Tryptophan. It is sometimes possible for the material contained within the vial of "Tryptophan, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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