Typical Testing Data/Standard Curve (for reference only)
()
Human Histamine ELISA Kit
Histamine ELISA
Reactivity
Human
Synonyms
Histamine; Histamine ELISA; Histamine elisa kit
Reactivity
Human
Samples
Plasma and urine
Assay Type
Quantitative Competitive
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Related Product Information for Histamine elisa kit
Intended Uses: Enzyme Immunoassay for the quantitative determination of Histamine in plasma and urine.
Principle of the Assay: In combination with the supplementary kit Histamine Release (for details contact your local supplier), the assay can be used for the measurement of histamine release in heparinized whole blood. In the first part of the procedure, Histamine is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated analyte concentrations in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-goat IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.
Background/Introduction: Histamine belongs to the biogenic amines and is synthesized by decarboxylation from the amino acid histidine. It is synthesized by mast cells, basophils, platelets, histaminergic neurons, and enterochromaffine cells, where it is stored intracellularly in vesicles and released on stimulation. Histamine acts by binding to its 4 receptors (H1R, H2R, H3R and H4R) on target cells in various tissues. It causes smooth muscle cell contraction, vasodilatation, increased vascular permeability and mucus secretion, tachycardia, alterations of blood pressure, and arrhythmias. In humans, histamine is one of the most important mediators and takes part in the initial phase of an anaphylactic reaction ("immediate type" allergy).
Principle of the Assay: In combination with the supplementary kit Histamine Release (for details contact your local supplier), the assay can be used for the measurement of histamine release in heparinized whole blood. In the first part of the procedure, Histamine is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated analyte concentrations in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-goat IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.
Background/Introduction: Histamine belongs to the biogenic amines and is synthesized by decarboxylation from the amino acid histidine. It is synthesized by mast cells, basophils, platelets, histaminergic neurons, and enterochromaffine cells, where it is stored intracellularly in vesicles and released on stimulation. Histamine acts by binding to its 4 receptors (H1R, H2R, H3R and H4R) on target cells in various tissues. It causes smooth muscle cell contraction, vasodilatation, increased vascular permeability and mucus secretion, tachycardia, alterations of blood pressure, and arrhythmias. In humans, histamine is one of the most important mediators and takes part in the initial phase of an anaphylactic reaction ("immediate type" allergy).
Product Categories/Family for Histamine elisa kit
Similar Products
Product Notes
The Human Histamine (Catalog #AAA494341) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494341 ELISA Kit recognizes Human Histamine. It is sometimes possible for the material contained within the vial of "Histamine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
