Human Cytokeratin 13 ELISA Kit | CK-13/KRT13 elisa kit

Human Cytokeratin 13 ELISA Kit

Cat. Num. AAA763999

• Reactivity: Human
• Synonyms: Cytokeratin 13; Human Cytokeratin 13 ELISA Kit; CK-13/KRT13/Cytokeratin-13/CK-13/Keratin-13/K13/Keratin; type I cytoskeletal 13; CK-13/KRT13 elisa kit

• Reactivity: Human
• Sequence Length: 420

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CK-13/KRT13 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human CK-13 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CK-13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CK-13 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CK-13 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 30377
• UniProt Accession #: P13646; Q53G54; Q6AZK5; Q8N240
• Molecular Weight: 45,867 Da
• NCBI Official Full Name: cytokeratin 13
• UniProt Protein Name: Keratin, type I cytoskeletal 13
• UniProt Gene Name: KRT13
• UniProt Synonym Gene Names: CK-13; K13
• UniProt Entry Name: K1C13_HUMAN

Uniprot Description

K13: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K4

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: intermediate filament cytoskeleton; keratin filament; nucleus

Molecular Function: structural molecule activity

Biological Process: cytoskeleton organization and biogenesis; response to radiation; tongue morphogenesis

Disease: White Sponge Nevus 2