Goat ALOX5 Polyclonal Antibody | anti-ALOX5 antibody

ALOX5 (Arachidonate 5-lipoxygenase, RP11-67C2.3, 5-LO, 5LPG, LOG5, arachidonic acid 5-lipoxygenase, leukotriene A4 synthase)

Cat. Num. AAA622085

• Gene Names: ALOX5; 5-LO; 5LPG; LOG5; 5-LOX; MGC163204
• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ALOX5; Polyclonal Antibody; ALOX5 (Arachidonate 5-lipoxygenase; RP11-67C2.3; 5-LO; 5LPG; LOG5; arachidonic acid 5-lipoxygenase; leukotriene A4 synthase); Anti -ALOX5 (Arachidonate 5-lipoxygenase; anti-ALOX5 antibody

• Host: Goat
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human Arachidonate 5-lipoxygenase. Species Crossreactivity: Canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA., 0.02% sodium azide.

• Applicable Applications for anti-ALOX5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32000; Western Blot: 0.3-1ug/ml. 85kD band observed in human bone marrow and rat stomach lysates.
• Immunogen: Synthetic peptide of Arachidonate 5-lipoxygenase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of Arachidonate 5-lipoxygenase in Rat Stomach lysate (35μg protein in RIPA buffer) using Arachidonate 5-lipoxygenase antibody (0.3μg/ml).)

Related Product Information for anti-ALOX5 antibody

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Product Categories/Family for anti-ALOX5 antibody

Antibodies; Abs to Leukotrienes

NCBI and Uniprot Product Information

• NCBI GI #: 4502057
• NCBI GeneID: 240
• NCBI Accession #: NP_000689.1
• NCBI GenBank Nucleotide #: NM_000698.2
• UniProt Accession #: P09917; Q5JQ14; Q59H13
• Molecular Weight: 77,983 Da
• NCBI Official Full Name: arachidonate 5-lipoxygenase
• NCBI Official Synonym Full Names: arachidonate 5-lipoxygenase
• NCBI Official Symbol: ALOX5
• NCBI Official Synonym Symbols: 5-LO; 5LPG; LOG5; 5-LOX; MGC163204
• NCBI Protein Information: arachidonate 5-lipoxygenase; OTTHUMP00000019502; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform
• UniProt Protein Name: Arachidonate 5-lipoxygenase
• Protein Family: Arachidonate 5-lipoxygenase
• UniProt Gene Name: ALOX5
• UniProt Synonym Gene Names: LOG5
• UniProt Entry Name: LOX5_HUMAN

NCBI Description

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq]

Uniprot Description

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.

Protein type: Nuclear envelope; Oxidoreductase; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; EC 1.13.11.34

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol

Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity

Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Asthma, Susceptibility To