Human Noradrenaline ELISA Kit

Noradrenaline (Plasma/Urine) ELISA

Cat. Num. AAA490394

• Reactivity: Human
• Applications: ELISA
• Synonyms: Noradrenaline; Noradrenaline (Plasma/Urine) ELISA; NORADRENALINE (PLASMA/URINE); Noradrenaline elisa kit

• Reactivity: Human

• Applicable Applications for Noradrenaline elisa kit: ELISA (EIA)
• Assay Type: Quantitative Competitive
• Preparation and Storage: Store at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Noradrenaline elisa kit

Intended Uses: Enzyme Immunoassay for the quantitative determination of noradrenaline (norepinephrine) in plasma and urine. Noradrenaline (norepinephrine) is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Principle of the Assay: Enzyme Immunoassay for the quantitative determination of noradrenaline (norepinephrine) in plasma and urine. Noradrenaline (norepinephrine) is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Background/Introduction: In humans the catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are neurotransmitters of the sympathetic nervous system and are involved in many physiological processes. The sympathetic nervous system sets the body to a heightened state of alert, also called as the body's fight-orflight response. In the human body the catecholamines and their metabolites indicate the adaptation of the body to acute and chronic stress.