Typical Testing Data/Standard Curve (for reference only)
ACTH elisa kit
ACTH Ultra Sensitive lum ELISA Kit (Mouse/Rat)
Synonyms
ACTH; ACTH Ultra Sensitive lum ELISA Kit (Mouse/Rat); ACTH elisa kit
Sequence Length
41
Samples
Human Serum, Plasma, Body Fluids, Tissue Lysates Or Cell Culture Supernates
Assay Type
Quantitative Sandwich
Detection Range
312pg/ml-20,000pg/ml
Sensitivity
<10pg/ml
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ACTH elisa kit
Intended Uses: For quantitative detection of RBP4 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants. For research use only.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-RBP4 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-RBP4 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RBP4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RBP4 can be calculated!!Background/Introduction: Retinol binding protein 4, plasma, also known as RBP4, is protein that in humans is encoded by the RBP4 gene. The RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. It belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. RBP4 has recently been described as an adipokine that contributes to insulin resistance in the AG4KO mouse model. It is secreted by adipocytes, and can act as a signal to other cells, when there is a decrease in plasma glucose concentration.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-RBP4 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-RBP4 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RBP4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RBP4 can be calculated!!Background/Introduction: Retinol binding protein 4, plasma, also known as RBP4, is protein that in humans is encoded by the RBP4 gene. The RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. It belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. RBP4 has recently been described as an adipokine that contributes to insulin resistance in the AG4KO mouse model. It is secreted by adipocytes, and can act as a signal to other cells, when there is a decrease in plasma glucose concentration.
Product Categories/Family for ACTH elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
ACTH, partial
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Product Notes
The ACTH (Catalog #AAA580004) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ACTH, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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