Mouse RBP-4 ELISA Kit | RBP-4 elisa kit

RBP-4 Mouse ELISA Kit

Cat. Num. AAA355449

• Gene Names: Rbp4; Rbp-4
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: RBP-4; RBP-4 Mouse ELISA Kit; Mouse RBP-4; RBP-4 elisa kit

• Reactivity: Mouse

• Applicable Applications for RBP-4 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of RBP4 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Assay Type: Sandwich
• Detection Range: 312 pg/ml-20,000 pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RBP-4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-RBP4 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-RBP4 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RBP4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RBP4 can be calculated.

Background: Retinol binding protein 4, plasma, also known as RBP4, is protein that in Mouse is encoded by the RBP4 gene. The RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. It belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. RBP4 has recently been described as an adipokine that contributes to insulin resistance in the AG4KO mouse model. It is secreted by adipocytes, and can act as a signal to other cells, when there is a decrease in plasma glucose concentration.

NCBI and Uniprot Product Information

• NCBI GI #: 226958688
• NCBI GeneID: 19662
• NCBI Accession #: NP_001152959.1
• NCBI GenBank Nucleotide #: NM_001159487.1
• UniProt Accession #: Q00724; P70357; Q566I5
• Molecular Weight: 23,206 Da
• NCBI Official Full Name: retinol-binding protein 4 isoform 1
• NCBI Official Synonym Full Names: retinol binding protein 4, plasma
• NCBI Official Symbol: Rbp4
• NCBI Official Synonym Symbols: Rbp-4
• NCBI Protein Information: retinol-binding protein 4; RBP; PRBP; plasma retinol-binding protein; retinol binding protein 4, cellular
• UniProt Protein Name: Retinol-binding protein 4
• UniProt Gene Name: Rbp4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_MOUSE

Uniprot Description

Function: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.

Subcellular location: Secreted.

Sequence similarities: Belongs to the calycin superfamily. Lipocalin family.