Testing Data
96-Well Ras Activation ELISA Kit
96-Well Ras Activation ELISA Kit (Chemiluminescent)
Synonyms
96-Well Ras Activation; 96-Well Ras Activation ELISA Kit (Chemiluminescent); 96-Well Ras Activation elisa kit
Preparation and Storage
Upon receipt, aliquot and store Raf-1 RBD at -80 degree C and avoid freeze/thaw. Aliquot and store the Anti-pan-Ras Antibody, Secondary Antibody, GTPgammaS, and GDP components at -20 degree C and avoid freeze/thaw. Store all other components at 4 degree C until their expiration dates.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for 96-Well Ras Activation elisa kit
Background/Introduction: Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Ras, a 21 kDa protein, regulates a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, Ras regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Ras binds specifically to the Ras-binding domain (RBD) of Raf-1 to control downstream signaling cascades. The most notable members of the Ras subfamily are H-Ras, N-Ras and K-Ras, mainly for being implicated in many types of cancer. 96-well Ras Activation ELISA Kit utilizes plate-bound, Raf-1 RBD to selectively isolate and pull-down the active forms of Ras (H-, K-, and N-Ras isoforms from human, mouse and rat) from purified samples or endogenous lysates. Subsequently, the captured GTP-Ras is detected by an Anti-pan-Ras Antibody and HRP conjugated secondary antibody. Finally, following the addition of Chemiluminescent Reagent, the reaction is then measured in a plate luminometer. 96-well Ras Activation ELISA Kit provides a simple and fast tool to monitor the activation of Ras. Each kit provides sufficient reagents to perform up to 96 assays.
Product Categories/Family for 96-Well Ras Activation elisa kit
References
1. Bar-Sagi D., and Hall A. (2000) Cell 103: 227-38.
2. de Rooij J., and Bos J. L. (1997) Oncogene 14: 623-5.
2. de Rooij J., and Bos J. L. (1997) Oncogene 14: 623-5.
Similar Products
Product Notes
The 96-Well Ras Activation (Catalog #AAA168173) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "96-Well Ras Activation, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
