Testing Data
RalGDS RBD Accessory Reagent
RalGDS RBD Agarose Beads
Synonyms
RalGDS RBD; RalGDS RBD Agarose Beads; RalGDS RBD accessory reagent
Activity
Product specifically interacts and precipitates GTP-bound Rap from cell lysate (Figure 2).
Quantity
800 uL of 50% Agarose slurry, 400 ug of murine RalGDSRBD (amino acid 726-823) in 1X PBS, 50% Glycerol
Preparation and Storage
-20 degree C. Shelf Life: 1 year from receipt under proper storage conditions; avoid multiple freeze thaw cycles
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for RalGDS RBD accessory reagent
• Pull down the active form of Rap
• Colored for easy visualization
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Rap, a 24 kDa protein of the Ras superfamily, regulates a variety of biological response pathways that include cell adhesion, proliferation, differentiation, and apoptosis. The Ras-like proteins Rap1 and Rap2 share 60% identity. Like other small GTPases, Rap regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In their active (GTP-bound) state, Rap1 and Rap2 bind specifically to the Rap-binding domain (RBD) of RalGDS to control downstream signaling cascades.
• Colored for easy visualization
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Rap, a 24 kDa protein of the Ras superfamily, regulates a variety of biological response pathways that include cell adhesion, proliferation, differentiation, and apoptosis. The Ras-like proteins Rap1 and Rap2 share 60% identity. Like other small GTPases, Rap regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In their active (GTP-bound) state, Rap1 and Rap2 bind specifically to the Rap-binding domain (RBD) of RalGDS to control downstream signaling cascades.
Product Categories/Family for RalGDS RBD accessory reagent
References
1. Kitayama, H., Sugimoto, Y., Matsuzaki, T., Ikawa, Y., and Noda, M. (1989) Cell 56, 77-84.
2. Matsubara, K., Kishida, S., Matsuura, Y., Kitayama, H., Noda, M., and Kikuchi, A. (1999) Oncogene
18, 1303-1312.
3. Bos, J. L., de Rooij, J., and Reedquist, K. A. (2001) Nature Rev. 2, 369-377.
4. Herrmann, C., Horn, G., Spaargaren, M., and Wittinghofer, A. (1996) J. Biol. Chem. 271, 6794-6800.
2. Matsubara, K., Kishida, S., Matsuura, Y., Kitayama, H., Noda, M., and Kikuchi, A. (1999) Oncogene
18, 1303-1312.
3. Bos, J. L., de Rooij, J., and Reedquist, K. A. (2001) Nature Rev. 2, 369-377.
4. Herrmann, C., Horn, G., Spaargaren, M., and Wittinghofer, A. (1996) J. Biol. Chem. 271, 6794-6800.
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Product Notes
The RalGDS RBD (Catalog #AAA168942) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RalGDS RBD, Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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