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Western Blot (WB) (FOXO6 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the FOXO6 antibody detected the FOXO6 protein (arrow).)

Rabbit anti-Mouse FOXO6 Polyclonal Antibody | anti-FOXO6 antibody

FOXO6 Antibody (Center)

Reactivity
Mouse
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FOXO6; Polyclonal Antibody; FOXO6 Antibody (Center); Forkhead box protein O6; anti-FOXO6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FOXO6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-181 amino acids from the Central region of human FOXO6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
154-181
Sequence Length
492
Applicable Applications for anti-FOXO6 antibody
Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(FOXO6 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the FOXO6 antibody detected the FOXO6 protein (arrow).)

Western Blot (WB) (FOXO6 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the FOXO6 antibody detected the FOXO6 protein (arrow).)

Immunofluorescence (IF)

(Fluorescent confocal image of U251 cell stained with FOXO6 Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXO6 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXO6 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)

Immunofluorescence (IF) (Fluorescent confocal image of U251 cell stained with FOXO6 Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXO6 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXO6 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Related Product Information for anti-FOXO6 antibody
FOXO6 is a transcriptional activator (By similarity).

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
50595
NCBI Official Full Name
Forkhead box protein O6
UniProt Protein Name
Forkhead box protein O6
Protein Family
UniProt Gene Name
FOXO6
UniProt Entry Name
FOXO6_HUMAN

Uniprot Description

FOXO6: Transcriptional activator.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent

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Product Notes

The FOXO6 foxo6 (Catalog #AAA9204816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 154-181. The FOXO6 Antibody (Center) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FOXO6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the FOXO6 foxo6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXO6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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