Rabbit anti-Human CCKAR Polyclonal Antibody | anti-CCKAR antibody

CCKAR, ID (CCKAR, CCKRA, Cholecystokinin receptor type A, Cholecystokinin-1 receptor) (FITC)

Cat. Num. AAA6282006

• Gene Names: CCKAR; CCK-A; CCKRA; CCK1-R
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCKAR; Polyclonal Antibody; ID (CCKAR; CCKRA; Cholecystokinin receptor type A; Cholecystokinin-1 receptor) (FITC); Cholecystokinin-1 receptor; anti-CCKAR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CCKAR antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CCKAR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-257 amino acids from the Central region of human CCKAR.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CCKAR Antibody (Center) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the CCKAR antibody detected the CCKAR protein (arrow).)

Related Product Information for anti-CCKAR antibody

This gene encodes a G-protein coupled receptor that binds non-sulfated members of the cholecystokinin (CCK) family of peptide hormones. This receptor is a major physiologic mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach. In the central and peripheral nervous system this receptor regulates satiety and the release of beta-endorphin and dopamine.

Product Categories/Family for anti-CCKAR antibody

Antibodies; Receptors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Cong, P., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (3), G742-G750 (2010): Tiwari, A.K., et al. Prog. Neuropsychopharmacol. Biol. Psychiatry (2010). In press: Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Park, S.Y., et al. J Neurogastroenterol Motil 16(1):71-76(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4502607
• NCBI GeneID: 886
• NCBI Accession #: NP_000721.1
• NCBI GenBank Nucleotide #: NM_000730.2
• UniProt Accession #: P32238; B2R9Z5
• Molecular Weight: 47,841 Da
• NCBI Official Full Name: cholecystokinin receptor type A
• NCBI Official Synonym Full Names: cholecystokinin A receptor
• NCBI Official Symbol: CCKAR
• NCBI Official Synonym Symbols: CCK-A; CCKRA; CCK1-R
• NCBI Protein Information: cholecystokinin receptor type A; CCK-AR; CCK-A receptor; cholecystokinin-1 receptor; cholecystokinin type-A receptor
• UniProt Protein Name: Cholecystokinin receptor type A
• Protein Family: Cholecystokinin receptor
• UniProt Gene Name: CCKAR
• UniProt Synonym Gene Names: CCKRA; CCK-A receptor; CCK-AR; CCK1-R
• UniProt Entry Name: CCKAR_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor that binds non-sulfated members of the cholecystokinin (CCK) family of peptide hormones. This receptor is a major physiologic mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach. In the central and peripheral nervous system this receptor regulates satiety and the release of beta-endorphin and dopamine. [provided by RefSeq, Jul 2008]

Uniprot Description

CCKAR: Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: cholecystokinin receptor activity; peptide binding

Biological Process: elevation of cytosolic calcium ion concentration; axonogenesis; digestion; forebrain development; regulation of hormone secretion; neuron migration; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); feeding behavior; response to nutrient; cellular response to hormone stimulus