Sheep E3/ubiquitin-protein ligase ELISA Kit | E3/UBPL elisa kit

Sheep E3/ubiquitin-protein ligase ELISA Kit

Cat. Num. AAA743549

• Gene Names: UBR5; DD5; EDD; HYD; EDD1
• Reactivity: Sheep
• Synonyms: E3/ubiquitin-protein ligase; Sheep E3/ubiquitin-protein ligase ELISA Kit; E3/UBPL elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for E3/UBPL elisa kit

Intended Uses: This E3/UBPL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep E3/UBPL. This ELISA kit for research use only!

Principle of the Assay: E3/UBPL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-E3/UBPL antibody and an E3/UBPL-HRP conjugate. The assay sample and buffer are incubated together with E3/UBPL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the E3/UBPL concentration since E3/UBPL from samples and E3/UBPL-HRP conjugate compete for the anti-E3/UBPL antibody binding site. Since the number of sites is limited, as more sites are occupied by E3/UBPL from the sample, fewer sites are left to bind E3/UBPL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E3/UBPL concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15147337
• NCBI GeneID: 51366
• NCBI Accession #: NP_056986.2
• NCBI GenBank Nucleotide #: NM_015902.5
• Molecular Weight: 309,224 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase UBR5 isoform 1
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3 component n-recognin 5
• NCBI Official Symbol: UBR5
• NCBI Official Synonym Symbols: DD5; EDD; HYD; EDD1
• NCBI Protein Information: E3 ubiquitin-protein ligase UBR5; progestin induced protein; progestin-induced protein; hyperplastic discs protein homolog; E3 identified by differential display; E3 ubiquitin-protein ligase, HECT domain-containing 1; E3 ubiquitin protein ligase, HECT domain containing, 1
• UniProt Protein Name: E3 ubiquitin-protein ligase UBR5
• UniProt Gene Name: UBR5
• UniProt Synonym Gene Names: EDD; EDD1; HYD; KIAA0896; hHYD
• UniProt Entry Name: UBR5_HUMAN

NCBI Description

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

UBR5: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Binds TOPBP1. Associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulates target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.

Protein type: Ubiquitin conjugating system; Ligase; Nuclear receptor co-regulator; EC 6.3.2.-; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: ubiquitin binding; protein binding; zinc ion binding; RNA binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; cell proliferation; protein polyubiquitination; progesterone receptor signaling pathway; positive regulation of protein import into nucleus, translocation; protein ubiquitination during ubiquitin-dependent protein catabolic process; DNA repair; response to DNA damage stimulus