SEMA6A recombinant protein

SEMA6A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120726

• Gene Names: SEMA6A; VIA; SEMA; HT018; SEMAQ; SEMA6A1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: SEMA6A; SEMA6A Protein; Human; Recombinant (His Tag); Human Semaphorin 6A/SEMA6A Protein (His Tag); HT018 Protein; SEMA Protein; SEMA6A1 Protein; SEMAQ Protein; VIA Protein; sema domain; transmembrane domain (TM); and cytoplasmic domain; (semaphorin) 6A; SEMA6A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr649

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human SEMA6A (NP_065847.1) extracellular domain (Met 1-Thr 649) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SEMA6A recombinant protein

Background: SEMA6A, the first identified class 6 semaphorin, is contributed to regulate vascular development and adult angiogenesis. SEMA6A could inhibit proliferation, migration, and invasion in different glioma cell lines. That SEMA6A may be a potential prognostic biomarker in the treatment of GBM. Among different semaphorins tested by reverse transcriptase-polymerase chain reaction in human immune cells, the expression of class 6 transmembrane semaphorin SEMA6A was restricted to dendritic cells (DCs).

References

Klostermann A., et al., 2000, J. Biol. Chem. 275:39647-53. Nagase T., et al., 2000, DNA Res. 7:65-73. Schmutz J., et al., 2004, Nature 431:268-74. Neufeld, G. et al., 2005, Front Biosci. 10 : 751-60. Fiore,R. et al., 2005, Mol Cell Biol. 25 (6):2310-9.

NCBI and Uniprot Product Information

• NCBI GI #: 11991660
• NCBI GeneID: 57556
• NCBI Accession #: NP_065847.1
• NCBI GenBank Nucleotide #: NM_020796.4
• UniProt Accession #: Q9H2E6; Q9P2H9
• Molecular Weight: 116,336 Da
• NCBI Official Full Name: semaphorin-6A isoform 2
• NCBI Official Synonym Full Names: semaphorin 6A
• NCBI Official Symbol: SEMA6A
• NCBI Official Synonym Symbols: VIA; SEMA; HT018; SEMAQ; SEMA6A1
• NCBI Protein Information: semaphorin-6A
• UniProt Protein Name: Semaphorin-6A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA6A
• UniProt Synonym Gene Names: KIAA1368; SEMAQ; Sema VIA; SEMA6A-1

NCBI Description

The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection (Leighton et al., 2001 [PubMed 11242070]).[supplied by OMIM, Feb 2011]

Uniprot Description

Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation ().