Rabbit E3/ubiquitin-protein ligase ELISA Kit | E3 / UBPL elisa kit

Rabbit E3/ubiquitin-protein ligase ELISA Kit

Cat. Num. AAA720974

• Gene Names: UBR5; DD5; EDD; HYD; EDD1
• Reactivity: Rabbit
• Synonyms: E3/ubiquitin-protein ligase; Rabbit E3/ubiquitin-protein ligase ELISA Kit; E3 / UBPL elisa kit

• Reactivity: Rabbit

• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for E3 / UBPL elisa kit

Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 15147337
• NCBI GeneID: 51366
• NCBI Accession #: NP_056986
• NCBI GenBank Nucleotide #: NM_015902.5
• UniProt Accession #: O95071; O94970; Q9NPL3; B2RP24
• Molecular Weight: 309,352 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase UBR5
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3 component n-recognin 5
• NCBI Official Symbol: UBR5
• NCBI Official Synonym Symbols: DD5; EDD; HYD; EDD1
• NCBI Protein Information: E3 ubiquitin-protein ligase UBR5; hHYD; progestin induced protein; progestin-induced protein; hyperplastic discs protein homolog; E3 identified by differential display; E3 ubiquitin-protein ligase, HECT domain-containing 1; E3 ubiquitin protein ligase, HECT domain containing, 1
• UniProt Protein Name: E3 ubiquitin-protein ligase UBR5
• UniProt Gene Name: UBR5
• UniProt Synonym Gene Names: EDD; EDD1; HYD; KIAA0896; hHYD
• UniProt Entry Name: UBR5_HUMAN

NCBI Description

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation

By similarity. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes. Ref.17 Ref.19 Ref.21

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Binds TOPBP1. Associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulate target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. Ref.2 Ref.13 Ref.17

Subcellular location: Nucleus.

Tissue specificity: Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.

Miscellaneous: A cysteine residue is required for ubiquitin-thioester formation.

Sequence similarities: Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.Contains 1 PABC domain.Contains 1 UBR-type zinc finger.

Sequence caution: The sequence BAA74919.3 differs from that shown. Reason: Erroneous initiation.