Mouse Mitochondrial uncoupling protein 3 (UCP3) ELISA Kit | UCP3 elisa kit

Mouse Mitochondrial uncoupling protein 3 (UCP3) ELISA Kit

Cat. Num. AAA7222741

• Gene Names: UCP3; SLC25A9
• Reactivity: Mouse
• Synonyms: Mitochondrial uncoupling protein 3 (UCP3); Mouse Mitochondrial uncoupling protein 3 (UCP3) ELISA Kit; UCP3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of UCP3. No significant cross-reactivity or interference between UCP3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between UCP3 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UCP3 elisa kit

Intended Uses: This UCP3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse UCP3. This ELISA kit for research use only!

Principle of the Assay: UCP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-UCP3 antibody and an UCP3-HRP conjugate. The assay sample and buffer are incubated together with UCP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UCP3 concentration since UCP3 from samples and UCP3-HRP conjugate compete for the anti-UCP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by UCP3 from the sample, fewer sites are left to bind UCP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UCP3 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13259546
• NCBI GeneID: 7352
• NCBI Accession #: NP_073714.1
• NCBI GenBank Nucleotide #: NM_022803.2
• Molecular Weight: 22,874 Da
• NCBI Official Full Name: mitochondrial uncoupling protein 3 isoform UCP3S
• NCBI Official Synonym Full Names: uncoupling protein 3 (mitochondrial, proton carrier)
• NCBI Official Symbol: UCP3
• NCBI Official Synonym Symbols: SLC25A9
• NCBI Protein Information: mitochondrial uncoupling protein 3; solute carrier family 25 member 9
• UniProt Protein Name: Mitochondrial uncoupling protein 3
• Protein Family: UBA domain-containing protein
• UniProt Gene Name: UCP3
• UniProt Synonym Gene Names: SLC25A9; UCP 3
• UniProt Entry Name: UCP3_HUMAN

NCBI Description

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.[provided by RefSeq, Nov 2008]

Uniprot Description

UCP3: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance. Defects in UCP3 may be involved in obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the mitochondrial carrier family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter; Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: protein binding; transporter activity; oxidative phosphorylation uncoupler activity

Biological Process: response to superoxide; response to glucocorticoid stimulus; respiratory gaseous exchange; fatty acid metabolic process; cellular response to hormone stimulus; response to insulin stimulus; mitochondrial transport; cellular metabolic process; proton transport; response to hypoxia; lipid metabolic process; response to activity; response to cold; response to nutrient; aging

Disease: Obesity