Macrophage Inflammatory protein-3 beta Active Protein | mMIP 3b active protein
Recombinant Mouse Macrophage Inflammatory protein-3 beta (CCL19)
Gene Names
Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
Macrophage Inflammatory protein-3 beta; Recombinant Mouse Macrophage Inflammatory protein-3 beta (CCL19); MIP 3b Mouse; Macrophage Inflammatory Protein-3 Beta Mouse Recombinant (CCL19); Small inducible cytokine A19; CCL19; Macrophage inflammatory protein 3 beta; MIP-3- beta; EBI1-ligand chemokine; ELC; Beta chemokine exodus-3; CK beta-11; chemokine (C-C motif) ligand 19; CKb11; MIP3B; MIP-3b; SCYA19; MGC34433; Epstein-Barr virus-induced molecule 1 ligand chemokine; mMIP 3b active protein
Host
E Coli
Purity/Purification
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
The CCL19 was lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Ala-Asn-Asp-Ala
Sequence Length
108
Solubility
It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-3b in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of MIP-3b as a Reference Standard.
Biological Activity
The Activity is calculated by the ability to chemoattract Human mature dendritic cells using a concentration range of 8-80 ng/ml.
Preparation and Storage
Lyophilized MIP-3b although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL19 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for mMIP 3b active protein
Description: Macrophage Inflammatory Protein-3 beta Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9216 Dalton. The MIP-3b is purified by proprietary chromatographic techniques.
Introduction: Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). CCL19 is expressed abundantly in thymus and lymph nodes, with moderate levels in trachea and colon and low levels in stomach, small intestine, lung, kidney and spleen. The gene for CCL19 is located on human chromosome 9. This chemokine elicits its effects on its target cells by binding to the chemokine receptor chemokine receptor CCR7. It attracts certain cells of the immune system, including dendritic cells and antigen-engaged B cells.
Introduction: Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). CCL19 is expressed abundantly in thymus and lymph nodes, with moderate levels in trachea and colon and low levels in stomach, small intestine, lung, kidney and spleen. The gene for CCL19 is located on human chromosome 9. This chemokine elicits its effects on its target cells by binding to the chemokine receptor chemokine receptor CCR7. It attracts certain cells of the immune system, including dendritic cells and antigen-engaged B cells.
Product Categories/Family for mMIP 3b active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,911 Da
NCBI Official Full Name
C-C motif chemokine 19
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 19
NCBI Official Symbol
Ccl19
NCBI Official Synonym Symbols
ELC; CKb11; MIP3B; Scya19; exodus-3
NCBI Protein Information
C-C motif chemokine 19; EBI-1 ligand chemokine; EBI1 ligand chemokine; EBI1-ligand chemokine; EBV-induced molecule 1 ligand chemokine; chemokine CCL19; epstein-Barr virus-induced molecule 1 ligand chemokine; small inducible cytokine A19; small-inducible cytokine A19
UniProt Protein Name
C-C motif chemokine 19
UniProt Gene Name
Ccl19
UniProt Synonym Gene Names
Elc; Scya19; EBI1 ligand chemokine; ELC
UniProt Entry Name
CCL19_MOUSE
Uniprot Description
CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide
Cellular Component: cell; intracellular; cytosol; external side of plasma membrane
Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding
Biological Process: positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; positive regulation of JNK cascade; dendritic cell chemotaxis; positive regulation of phosphoinositide 3-kinase activity; establishment of T cell polarity; positive regulation of NF-kappaB import into nucleus
Research Articles on mMIP 3b
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Product Notes
The mMIP 3b ccl19 (Catalog #AAA142048) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Ala-As n-Asp-Ala. It is sometimes possible for the material contained within the vial of "Macrophage Inflammatory protein-3 beta, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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