Mouse CCL19/MIP-3 beta PicoKine ELISA Kit | Ccl19 elisa kit

Mouse CCL19/MIP-3 beta PicoKine ELISA Kit

Cat. Num. AAA177585

• Gene Names: Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3
• Reactivity: Mouse
• Synonyms: CCL19/MIP-3 beta PicoKine; Mouse CCL19/MIP-3 beta PicoKine ELISA Kit; C-C motif chemokine 19; Beta chemokine exodus 3; Beta-chemokine exodus-3; C C chemokine ligand 19; CC chemokine ligand 19; CCL19; CCL19_HUMAN; Chemokine (C C motif) ligand 19; Chemokine (CC motif) ligand 19; Chemokine CC Motif Ligand 19; CK beta 11; CK beta-11; CKb11; EBI1 ligand chemokine; ELC; Epstein-Barr virus-induced molecule 1 ligand chemokine; Exodus 3; Macrophage inflammatory protein 3 beta; MGC34433; MIP 3 beta; MIP-3-beta; MIP-3B; MIP3 beta; MIP3B; OTTHUMP00000000531; SCYA19; Small inducible cytokine A19; Small-inducible cytokine A19; chemokine (C-C motif) ligand 19; Ccl19 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CCL19
• Sequence Length: 108

• Assay Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of mouse CCL19 in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: E Coli, G26-S108
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml mouse CCL19 standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CCL19 standard solution and each sample is measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|91|286|592|105|379|645; Standard deviation|3.82|14.30|34.93|6.51|28.05|52.25; CV(%)|4.2|5.0|5.9|6.2|7.4|8.1
• Sample Data: Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000; O.D.|0.011|0.037|0.073|0.122|0.270|0.644|1.442|2.317
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse CCL19 PicoKine ELISA Kit standard curve )

Related Product Information for Ccl19 elisa kit

Principle of the assay: Mouse CCL19 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL19 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: G26-S108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL19 amount of sample captured in plate.

Background: Chemokine(C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was mapped to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

NCBI and Uniprot Product Information

• NCBI GI #: 6755422
• NCBI GeneID: 24047
• NCBI Accession #: NP_036018.1
• NCBI GenBank Nucleotide #: NM_011888.2
• UniProt Accession #: O70460
• Molecular Weight: 11,911 Da
• NCBI Official Full Name: C-C motif chemokine 19
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 19
• NCBI Official Symbol: Ccl19
• NCBI Official Synonym Symbols: ELC; CKb11; MIP3B; Scya19; exodus-3
• NCBI Protein Information: C-C motif chemokine 19
• UniProt Protein Name: C-C motif chemokine 19
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl19
• UniProt Synonym Gene Names: Elc; Scya19; EBI1 ligand chemokine; ELC
• UniProt Entry Name: CCL19_MOUSE

Uniprot Description

CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Cellular Component: external side of plasma membrane; extracellular space; intracellular

Molecular Function: CCR chemokine receptor binding; CCR10 chemokine receptor binding; chemokine activity; chemokine receptor binding

Biological Process: dendritic cell chemotaxis; establishment of T cell polarity; G-protein coupled receptor protein signaling pathway; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; myeloid dendritic cell chemotaxis; neutrophil chemotaxis; positive regulation of GTPase activity; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; release of sequestered calcium ion into cytosol