Human Fibroblast Growth Factor Receptor 4 (FGFR4) RTU ELISA Kit | FGFR4 rtu elisa kit

Human Fibroblast Growth Factor Receptor 4 (FGFR4) ELISA Kit

Cat. Num. AAA459329

• Gene Names: FGFR4; TKF; JTK2; CD334
• Reactivity: Human
• Synonyms: Fibroblast Growth Factor Receptor 4 (FGFR4); Human Fibroblast Growth Factor Receptor 4 (FGFR4) ELISA Kit; CD334; JTK2; TKF; FGFR4 rtu elisa kit

• Reactivity: Human
• Sequence Length: 734

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.041ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGFR4 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FGFR4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FGFR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FGFR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FGFR4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 634743314
• NCBI GeneID: 2264
• NCBI Accession #: NP_001278909.1
• NCBI GenBank Nucleotide #: NM_001291980.1
• UniProt Accession #: P22455; O43785; Q14309; Q71TW8; Q8TDA0; Q96KE5; G3JVM2; G3JVM5; G3JVM7; G3JVM9
• Molecular Weight: 64,640 Da
• NCBI Official Full Name: fibroblast growth factor receptor 4 isoform 3
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 4
• NCBI Official Symbol: FGFR4
• NCBI Official Synonym Symbols: TKF; JTK2; CD334
• NCBI Protein Information: fibroblast growth factor receptor 4
• UniProt Protein Name: Fibroblast growth factor receptor 4
• Protein Family: Fibroblast growth factor receptor
• UniProt Gene Name: FGFR4
• UniProt Synonym Gene Names: JTK2; TKF; FGFR-4

NCBI Description

The protein encoded by this gene is a tyrosine kinase and cell surface receptor for fibroblast growth factors. The encoded protein is involved in the regulation of several pathways, including cell proliferation, cell differentiation, cell migration, lipid metabolism, bile acid biosynthesis, vitamin D metabolism, glucose uptake, and phosphate homeostasis. This protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. [provided by RefSeq, Aug 2017]

Uniprot Description

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.