Mouse CCL19 ELISA Kit | CCL19 elisa kit

Mouse CCL19 ELISA Kit

Cat. Num. AAA8248422

• Gene Names: Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3
• Reactivity: Mouse
• Synonyms: CCL19; Mouse CCL19 ELISA Kit; ELC; MIP3B; SCYA19; C-C motif chemokine 19; Beta-chemokine exodus-3; CK beta-11; Epstein-Barr virus-induced molecule 1 ligand chemokine; EBI1 ligand chemokine; Macrophage inflammatory protein 3 beta; MIP-3-beta; Small-inducible cytokine A19; CCL19 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse CCL19 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CCL19 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 108

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL19 elisa kit

Principle of the Assay: The Mouse CCL19 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CCL19 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse CCL19 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL19 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CCL19 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL19 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Chemokine(C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was mapped to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

NCBI and Uniprot Product Information

• NCBI GI #: 6755422
• NCBI GeneID: 24047
• NCBI Accession #: NP_036018.1
• NCBI GenBank Nucleotide #: NM_011888.2
• UniProt Accession #: O70460
• Molecular Weight: 11,911 Da
• NCBI Official Full Name: C-C motif chemokine 19
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 19
• NCBI Official Symbol: Ccl19
• NCBI Official Synonym Symbols: ELC; CKb11; MIP3B; Scya19; exodus-3
• NCBI Protein Information: C-C motif chemokine 19
• UniProt Protein Name: C-C motif chemokine 19
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl19
• UniProt Synonym Gene Names: Elc; Scya19; EBI1 ligand chemokine; ELC
• UniProt Entry Name: CCL19_MOUSE

Uniprot Description

CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: external side of plasma membrane; extracellular space

Molecular Function: CCR chemokine receptor binding; CCR10 chemokine receptor binding; CCR7 chemokine receptor binding; chemokine activity; chemokine receptor binding

Biological Process: activation of JNK activity; cell maturation; dendritic cell chemotaxis; establishment of T cell polarity; formation of immunological synapse; G-protein coupled receptor protein signaling pathway; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; myeloid dendritic cell chemotaxis; neutrophil chemotaxis; positive regulation of chemotaxis; positive regulation of dendritic cell antigen processing and presentation; positive regulation of endocytosis; positive regulation of GTPase activity; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; positive regulation of receptor-mediated endocytosis; positive regulation of T cell proliferation; positive regulation of T-helper 1 cell differentiation; positive regulation of tumor necrosis factor production; release of sequestered calcium ion into cytosol; T cell costimulation