SDS-Page
SlyD active protein
SlyD,1-196aa, E Coli, E Coli (Bioactivity Validated)
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
SlyD; 1-196aa; E Coli; E Coli (Bioactivity Validated); FKBP-type peptidyl-prolyl cis-trans isomerase; FKBP-type peptidyl-prolyl cis-trans isomerase FKBP type peptidyl prolyl cis trans isomerase slyD; Histidine rich protein; PPIase; Rotamase; WHP.; SlyD active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid in 20mM Tris pH 7.5
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
Applicable Applications for SlyD active protein
SDS-PAGE
Antigen Species
E Coli
Biological Activity
Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmol of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for SlyD active protein
SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E Coli slyD was expressed in E Coli and purified by conventional chromatography.
Product Categories/Family for SlyD active protein
References
Zhang JW., et al. (2007) J Bacteriol. 189 (21):7942-4.; Scholz C., et al. (2006) Biochemistry. 45 (1):20-33.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21 kDa (196 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
FKBP-type peptidyl-prolyl cis-trans isomerase
Protein Family
Similar Products
Product Notes
The SlyD (Catalog #AAA203183) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SlyD can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SlyD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH. It is sometimes possible for the material contained within the vial of "SlyD, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
