SDS-Page
Cyclophilin G Active Protein | PPIG active protein
Cyclophilin G, 1-175aa, Human, His tag,E Coli (Bioactivity Validated)
Gene Names
PPIG; CYP; SRCyp; SCAF10; CARS-Cyp
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Cyclophilin G; 1-175aa; Human; His tag; E Coli (Bioactivity Validated); Peptidyl-prolyl cis-trans isomerase G; PPIase G; Rotamase G; CASP10; PPIG; peptidylprolyl isomerase G (cyclophilin G); CARS-Cyp; SRCyp; SCAF10; SR-related CTD-associated factor 10; ; PPIG active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGIKVQRPRC FFDIAINNQP AGRVVFELFS DVCPKTCENF RCLCTGEKGT GKSTQKPLHY KSCLFHRVVK DFMVQGGDFS EGNGRGGESI YGGFFEDESF AVKHNKEFLL SMANRGKDTN GSQFFITTKP TPHLDGHHVV FGQVISGQEV VREIENQKTD AASKPFAEVR ILSCG
Sequence Length
357
Applicable Applications for PPIG active protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Biological Activity
Specific activity is > 600 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for PPIG active protein
Peptidyl-prolyl cis-trans isomerase G (PPIG), also known as Cyclophilin G, is a member of peptidyl-prolyl cis-trans isomerase family (PPIases). This protein catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and is implicated in the folding, transport, and assembly of proteins. It is localized to the nuclear speckles, a nuclear compartment rich in splicing factors, and interacts with the splicing factors SC35 and pinin. Cyclophilin G also may play an important role in the regulation of pre-mRNA splicing. Recombinant human Cyclophilin G, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for PPIG active protein
References
Modjtahedi N., et al. (2004). J Biol Chem. 279 (21):22322-30 ; Ouyang P., et al. (2004). Biochem Biophys Re Commun. 321 (3): 638-47 ;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21.6 kDa (195aa), confirmed by MALDI-TOF.
NCBI Official Full Name
PPIG protein
NCBI Official Synonym Full Names
peptidylprolyl isomerase G
NCBI Official Symbol
PPIG
NCBI Official Synonym Symbols
CYP; SRCyp; SCAF10; CARS-Cyp
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase G
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase G
Protein Family
UniProt Gene Name
PPIG
UniProt Synonym Gene Names
PPIase G; Peptidyl-prolyl isomerase G; CARS-Cyp; CARS-cyclophilin; SR-cyclophilin; SR-cyp; SRcyp
Uniprot Description
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.
Research Articles on PPIG
Similar Products
Product Notes
The PPIG ppig (Catalog #AAA203587) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Cyclophilin G can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PPIG ppig for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGIKVQRPRC FFDIAINNQP AGRVVFELFS DVCPKTCENF RCLCTGEKGT GKSTQKPLHY KSCLFHRVVK DFMVQGGDFS EGNGRGGESI YGGFFEDESF AVKHNKEFLL SMANRGKDTN GSQFFITTKP TPHLDGHHVV FGQVISGQEV VREIENQKTD AASKPFAEVR ILSCG. It is sometimes possible for the material contained within the vial of "Cyclophilin G, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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