Ppif active protein

Ppif, 30-206 aa, Rat, His-tag, E Coli (Bioactivity Validated)

Cat. Num. AAA206502

• Gene Names: Ppif; CypD; CyP-D; PPIase
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Ppif; 30-206 aa; Rat; His-tag; E Coli (Bioactivity Validated); Peptidyl-prolyl cis-trans isomerase F; mitochondrial; CyP-D; CypD; PPIase; Ppif active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol, 1mM DTT
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF GSKSGKTSKK IVITDCGQLS
• Sequence Length: 206

• Applicable Applications for Ppif active protein: SDS-PAGE
• Antigen Species: Rat
• Tag Information: His-tag
• Biological Activity: Specific activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Ppif active protein

Ppif also known as Peptidyl-prolyl cis-trans isomerase F, mitochondrial. Ppif accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis the requirement of the PPIase activity for this function is debated. Recombinant rat Ppif was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for Ppif active protein

Enzymes & Proteases; Metabolism

References

Connern C.P., et al. (1992) Biochem. J. 284:381-385.; Lin DT., et al. (2002) J Biol Chem. 23:277 (34):31134-41.

NCBI and Uniprot Product Information

• NCBI GI #: 26892289
• NCBI GeneID: 282819
• NCBI Accession #: NP_758443
• NCBI GenBank Nucleotide #: NM_172243.1
• UniProt Accession #: P29117
• Molecular Weight: 21.2Da (200aa) confirmed by MALDI-TOF
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase F, mitochondrial
• NCBI Official Synonym Full Names: peptidylprolyl isomerase F
• NCBI Official Symbol: Ppif
• NCBI Official Synonym Symbols: CypD; CyP-D; PPIase
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase F, mitochondrial
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase F, mitochondrial
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: Ppif
• UniProt Synonym Gene Names: PPIase F; CyP-D; CypD

NCBI Description

mitochondrial isozyme of peptidyl-prolyl cis-trans-isomerase; enzyme activity is inhibited by cyclosporin A [RGD, Feb 2006]

Uniprot Description

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F1F0 ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis.