FKBP14 active protein

Human Recombinant FKBP14

Cat. Num. AAA844945

• Gene Names: FKBP14; EDSKMH; FKBP22; IPBP12
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: FKBP14; Human Recombinant FKBP14; Peptidyl-prolyl cis-trans isomerase FKBP14; FKBP22; FKBP14 active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 1 mg/ml solution in PBS (pH 7.4) containing 10% glycerol.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MALIPEPEVK IEVLQKPFIC HRKTKGGDLM LVHYEGYLEK DGSLFHSTHK HNNGQPIWFT LGILEALKGW DQGLKGMCVG EKRKLIIPPA LGYGKEGKGK IPPESTLIFN IDLLEIRNGP RSHESFQEMD LNDDWKLSKD EVKAYLKKEF EKHGAVVNES HHDALVEDIF DKEDEDKDGF ISAREFTYKH DEL
• Sequence Length: 211

• Applicable Applications for FKBP14 active protein: SDS-PAGE
• Biological Activity: Specific activity is > 240 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 umole of Suc-AAFP-pNA per minute at 25 degree C in Tris HCl pH 8.0 using chymotrypsin.
• Unit Definition: Specific activity is defined as the amount of enzyme that cleaves 1 umole of Suc-AAFP-pNA per minute at 25 degree C in Tris HCl pH 8.0 using chymotrypsin.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for FKBP14 active protein

Background: FKBP14 enzyme accelerates the folding of proteins during protein synthesis. It contains 2 EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 significantly decreases peptidyl prolyl cis-trans isomerase activity, therefore suggesting that the PPIase FKBP-type domain must be located at the N-terminus.

Product Categories/Family for FKBP14 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)  

NCBI and Uniprot Product Information

• NCBI GI #: 8923659
• NCBI GeneID: 55033
• NCBI Accession #: NP_060416.1
• NCBI GenBank Nucleotide #: NM_017946.3
• UniProt Accession #: Q9NWM8
• Molecular Weight: 24.2 kDa (213 aa, 20-211 aa + NT His Tag)
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP14
• NCBI Official Synonym Full Names: FK506 binding protein 14, 22 kDa
• NCBI Official Symbol: FKBP14
• NCBI Official Synonym Symbols: EDSKMH; FKBP22; IPBP12
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP14
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP14
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: FKBP14
• UniProt Synonym Gene Names: FKBP22; PPIase FKBP14; 22 kDa FKBP; FKBP-22; FKBP-14
• UniProt Entry Name: FKB14_HUMAN

NCBI Description

The protein encoded by this gene is a member of the FK506-binding protein family of peptidyl-prolyl cis-trans isomerases. The encoded protein is found in the lumen of the endoplasmic reticulum, where it is thought to accelerate protein folding. Defects in this gene are a cause of a type of Ehlers-Danlos syndrome (EDS). Both a protein-coding variant and noncoding variants are transcribed from this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

FKBP14: PPIases accelerate the folding of proteins during protein synthesis. Defects in FKBP14 are the cause of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss (EDSKMH). A syndrome with features of Ehlers-Danlos syndrome types VIA and VIB on the one hand, and the collagen VI- related congenital myopathies Ullrich congenital muscular dystrophy and Bethlem myopathy on the other hand. Clinically, this disorder is characterized by the following features: severe generalized hypotonia at birth with marked muscle weakness that improve in infancy; early-onset progressive kyphoscoliosis; joint hypermobility without contractures; hyperelastic skin with follicular hyperkeratosis, easy bruising, and occasional abnormal scarring; myopathy as confirmed by muscle MRI, histology, and electron microscopy; hearing impairment, which is predominantly sensorineural; and a normal ratio of lysyl pyridinoline to hydroxylysyl pyridinoline (LP/HP) in urine.

Protein type: Isomerase; EC 5.2.1.8; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen

Molecular Function: peptidyl-prolyl cis-trans isomerase activity; FK506 binding; calcium ion binding

Biological Process: unfolded protein response, activation of signaling protein activity; protein peptidyl-prolyl isomerization; cellular protein metabolic process; unfolded protein response

Disease: Ehlers-danlos Syndrome With Progressive Kyphoscoliosis, Myopathy, And Hearing Loss