NME2 active protein

NME2, human recombinant

Cat. Num. AAA844216

• Gene Names: NME1-NME2; NMELV; NM23-LV
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: NME2; human recombinant; Nucleoside diphosphate kinase B; NDPK-B; NDPKB; NM23-H2; NM23B.; NME2 active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 1 mM DTT; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE
• Sequence Length: 267

• Applicable Applications for NME2 active protein: SDS-PAGE
• Biological Activity: Specific activity is > 340 units/ml, in which one unit will convert 1.0 umole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25 degree C in a coupled system with PK/LDH.
• Results: Specific activity is > 340 units/ml
• Unit Definition: One unit will convert 1.0 umole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25 degree C in a coupled system with PK/LDH.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for NME2 active protein

Background: NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for NME2 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 66392203
• NCBI GeneID: 654364
• NCBI Accession #: NP_001018146.1
• NCBI GenBank Nucleotide #: NM_001018136.2
• UniProt Accession #: P22392; Q1WM23; Q6LCT6; A8MWA3
• Molecular Weight: 17.2 kDa (152 aa, 1-152 aa)
• NCBI Official Full Name: NME1-NME2 protein
• NCBI Official Synonym Full Names: NME1-NME2 readthrough
• NCBI Official Symbol: NME1-NME2
• NCBI Official Synonym Symbols: NMELV; NM23-LV
• NCBI Protein Information: NME1-NME2 protein
• UniProt Protein Name: Nucleoside diphosphate kinase B
• UniProt Gene Name: NME2
• UniProt Synonym Gene Names: NM23B; NDK B; NDP kinase B
• UniProt Entry Name: NDKB_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring NME1 and NME2 genes. The significance of this read-through transcription and the function of the resulting protein product have not yet been determined. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Uniprot Description

NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.4.6; Nucleotide Metabolism - purine; Nuclear receptor co-regulator; Kinase, nucleoside diphosphate; EC 2.7.13.3; Kinase, other; Nucleotide Metabolism - pyrimidine; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: ruffle; centrosome; focal adhesion; mitochondrion; membrane; perinuclear region of cytoplasm; lamellipodium; cytoplasm; intermediate filament; cytosol; nucleus

Molecular Function: gamma-tubulin binding; protein binding; metal ion binding; protein histidine kinase activity; intermediate filament binding; nucleoside diphosphate kinase activity; transcription factor activity; protein kinase binding; single-stranded DNA binding; ATP binding

Biological Process: integrin-mediated signaling pathway; GTP biosynthetic process; peptidyl-histidine phosphorylation; response to cAMP; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; UTP biosynthetic process; negative regulation of myeloid leukocyte differentiation; hippocampus development; nucleoside diphosphate phosphorylation; response to testosterone stimulus; CTP biosynthetic process; regulation of transcription, DNA-dependent; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; positive regulation of epithelial cell proliferation; regulation of epidermis development; negative regulation of apoptosis; response to amine stimulus; purine nucleotide metabolic process; positive regulation of keratinocyte differentiation