Testing Data
(Detection limit for recombinant GST tagged AIF1 is 0.03ng/ml using MBS609673 as a capture antibody.)
Mouse anti-Human AIF1 Monoclonal Antibody | anti-AIF1 antibody
AIF1 (Allograft Inflammatory Factor 1, AIF-1, DASS-82G15.2, G1, IBA1, IRT-1, Em:AF129756.17, Interferon gamma Responsive Transcript, IRT-1, Ionized Calcium-binding Adapter Molecule, Protein G1) (AP)
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified
Synonyms
AIF1; Monoclonal Antibody; AIF1 (Allograft Inflammatory Factor 1; AIF-1; DASS-82G15.2; G1; IBA1; IRT-1; Em:AF129756.17; Interferon gamma Responsive Transcript; Ionized Calcium-binding Adapter Molecule; Protein G1) (AP); anti-AIF1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2A2-B6
Specificity
Recognizes human AIF1.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Sequence Length
147
Applicable Applications for anti-AIF1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant protein corresponding to aa1-147 from human AIF1 with GST tag. MW of the GST tag alone is 26kD.
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(Detection limit for recombinant GST tagged AIF1 is 0.03ng/ml using MBS609673 as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged AIF1 is 0.03ng/ml using MBS609673 as a capture antibody.)
Western Blot (WB)
(Western Blot analysis of AIF1 expression in K-562 usingMBS609673.)
Western Blot (WB)
(Western Blot analysis of AIF1 expression in K-562 usingMBS609673.)
Related Product Information for anti-AIF1 antibody
AIF-1 is a 17kD IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis. Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region.
Product Categories/Family for anti-AIF1 antibody
References
Autieri MV, Agrawal N. IRT-1, a novel interferon-gamma-responsive transcript encoding a growth- suppressing basic leucine zipper protein. J Biol Chem. 1998 Jun 12;273(24):14731-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Allograft inflammatory factor 1
NCBI Official Synonym Full Names
allograft inflammatory factor 1
NCBI Official Symbol
AIF1
NCBI Official Synonym Symbols
IBA1; IRT1; AIF-1; IRT-1
NCBI Protein Information
allograft inflammatory factor 1
Protein Family
NCBI Description
This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]
Research Articles on AIF1
Similar Products
Product Notes
The AIF1 (Catalog #AAA6129715) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AIF1 (Allograft Inflammatory Factor 1, AIF-1, DASS-82G15.2, G1, IBA1, IRT-1, Em:AF129756.17, Interferon gamma Responsive Transcript, IRT-1, Ionized Calcium-binding Adapter Molecule, Protein G1) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AIF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AIF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AIF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
