Rabbit anti-Human, Mouse NME2 Polyclonal Antibody | anti-NME2 antibody

NME2 Antibody

Cat. Num. AAA9407020

• Gene Names: NME1-NME2; NMELV; NM23-LV
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: NME2; Polyclonal Antibody; NME2 Antibody; PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2; anti-NME2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total NME2 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 2.8 mg/ml (varies by lot)
• Sequence Length: 267

• Applicable Applications for anti-NME2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:1000-1:5000; Immunohistochemistry: 1:30-1:150
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to residues near the C terminal of human NME/NM23 nucleoside diphosphate kinase 2
• Target Name: NME2
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 12%SDS-PAGE Lysates (from left to right): Hela, NIH/3T3, lncap and 293T cell, mouse brain tissue, A549 and Jurkat cell Amount of lysate: 40ug per lane Primary antibody: 1/300 dilution Secondary antibody dilution: 1/8000Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/40.)

Related Product Information for anti-NME2 antibody

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product.

Product Categories/Family for anti-NME2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 66392203
• NCBI GeneID: 654364
• NCBI Accession #: NP_001018146.1
• NCBI GenBank Nucleotide #: NM_001018136.2
• UniProt Accession #: P22392; Q1WM23; Q6LCT6; A8MWA3
• Molecular Weight: 17kDa
• NCBI Official Full Name: NME1-NME2 protein
• NCBI Official Synonym Full Names: NME1-NME2 readthrough
• NCBI Official Symbol: NME1-NME2
• NCBI Official Synonym Symbols: NMELV; NM23-LV
• NCBI Protein Information: NME1-NME2 protein
• UniProt Protein Name: Nucleoside diphosphate kinase B
• UniProt Gene Name: NME2
• UniProt Synonym Gene Names: NM23B; NDK B; NDP kinase B
• UniProt Entry Name: NDKB_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring NME1 and NME2 genes. The significance of this read-through transcription and the function of the resulting protein product have not yet been determined. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Uniprot Description

NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Kinase, other; Nucleotide Metabolism - pyrimidine; Nuclear receptor co-regulator; EC 2.7.13.3; EC 2.7.4.6; Kinase, nucleoside diphosphate; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: ruffle; centrosome; focal adhesion; mitochondrion; membrane; lamellipodium; perinuclear region of cytoplasm; cytoplasm; intermediate filament; nucleus; cytosol

Molecular Function: protein binding; gamma-tubulin binding; metal ion binding; protein histidine kinase activity; intermediate filament binding; nucleoside diphosphate kinase activity; protein kinase binding; transcription factor activity; ATP binding; single-stranded DNA binding

Biological Process: integrin-mediated signaling pathway; GTP biosynthetic process; peptidyl-histidine phosphorylation; response to cAMP; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; UTP biosynthetic process; negative regulation of myeloid leukocyte differentiation; hippocampus development; nucleoside diphosphate phosphorylation; response to testosterone stimulus; CTP biosynthetic process; regulation of transcription, DNA-dependent; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; regulation of epidermis development; positive regulation of epithelial cell proliferation; positive regulation of keratinocyte differentiation; purine nucleotide metabolic process; negative regulation of apoptosis; response to amine stimulus