Human Toll-like receptor 10 ELISA Kit | TLR10 elisa kit

Human Toll-like receptor 10 ELISA Kit

Cat. Num. AAA289704

• Gene Names: TLR10; CD290
• Reactivity: Human
• Synonyms: Toll-like receptor 10; Human Toll-like receptor 10 ELISA Kit; TLR10; UNQ315/PRO358; CD290; TLR10 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Toll-like receptor 10. No significant cross-reactivity or interference was observed.
• Sequence Length: 811

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/mL
• Sensitivity: 39 pg/mL
• Intra-Assay CV: <=8.3%
• Inter-Assay CV: <=9.5%
• Recovery: 101%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR10 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Toll-like receptor 10 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll-like receptor 10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Toll-like receptor 10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Toll-like receptor 10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Toll-like receptor 10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62865621
• NCBI GeneID: 81793
• NCBI Accession #: NP_001017388.1
• NCBI GenBank Nucleotide #: NM_001017388.2
• UniProt Accession #: Q9BXR5; Q32MI7; Q32MI8; Q5FWG4; Q6UXL3; A8K7L1; B3Y668; D1CS21; D1CS22
• Molecular Weight: 94,564 Da
• NCBI Official Full Name: toll-like receptor 10 isoform a
• NCBI Official Synonym Full Names: toll like receptor 10
• NCBI Official Symbol: TLR10
• NCBI Official Synonym Symbols: CD290
• NCBI Protein Information: toll-like receptor 10
• UniProt Protein Name: Toll-like receptor 10
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR10
• UniProt Entry Name: TLR10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is most highly expressed in lymphoid tissues such as spleen, lymph node, thymus, and tonsil. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

TLR10: Participates in the innate immune response to microbial agents. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Belongs to the Toll-like receptor family.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: integral to membrane; membrane; plasma membrane

Molecular Function: transmembrane receptor activity

Biological Process: immune response; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; positive regulation of inflammatory response; regulation of cytokine secretion; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway