SDS-Page
Egf active protein
Egf, 977-1029aa, Mouse, His-tag, E Coli (Bioactivity Validated)
Gene Names
Egf; AI790464
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Egf; 977-1029aa; Mouse; His-tag; E Coli (Bioactivity Validated); Pro-epidermal growth factor isoform1; AI790464; Egf active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl (pH 8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR
Sequence Length
1217
Applicable Applications for Egf active protein
SDS-PAGE
Antigen Species
Mouse
Tag Information
His-tag
Endotoxin Level
< 0.2 EU per 1ug of protein (determined by LAL method)
Biological Activity
Measured in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 for this effect less or equal to 1ng/ml.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for Egf active protein
Egf, also known as Pro-epidermal growth factor isoform1, is a 8.6 kDa globular protein containing 77 amino acids residues, including 3 intra-molecular disulfide bonds. Egf is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, Egf has been shown to inhibit gastric secretion, and to be involved in wound healing. Egf signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant mouse Egf protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional column chromatography
Product Categories/Family for Egf active protein
References
Riese., et al (1998) Bioessays. 20 (1):41-8.; Cohen S (1983) Cancer. 51 (10):1787-91.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
8.6 kDa (77aa) Confirmed by MALDI-TOF
NCBI Official Full Name
pro-epidermal growth factor isoform 1 preproprotein
NCBI Official Synonym Full Names
epidermal growth factor
NCBI Official Symbol
Egf
NCBI Official Synonym Symbols
AI790464
NCBI Protein Information
pro-epidermal growth factor
UniProt Protein Name
Pro-epidermal growth factor
Protein Family
UniProt Gene Name
Egf
UniProt Synonym Gene Names
EGF
NCBI Description
This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016]
Uniprot Description
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 ().
Research Articles on Egf
Similar Products
Product Notes
The Egf egf (Catalog #AAA206779) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Egf can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Egf egf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR. It is sometimes possible for the material contained within the vial of "Egf, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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