Egf recombinant protein

Human CellExp EGF, Fc Tag, Mouse recombinant

Cat. Num. AAA8400052

• Gene Names: Egf; AI790464
• Purity: >95% by SDS-PAGE
• Synonyms: EGF; Human CellExp EGF; Fc Tag; Mouse recombinant; Epidermal growth factor; URG; HOMG4; Egf recombinant protein

• Host: HEK293 cells
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Lyophilized powder. Lyophilized from 0.22um filtered solution in 50mM Tris, 100mM Glycine, 25mM Arginine, 150mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization
• Sequence: aaAsn977-Arg1029

• Gene Source: Mouse
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Reconstitution Instruction: Reconstitute in sterile deionized water to the desired protein concentration.
• Tag Note: This protein carries a human IgG1 Fc tag at the N-terminus.
• Preparation and Storage: Store at -20 degree C; Centrifuge the vial prior to opening.; Shelf life: ~12 months

SDS-Page

Testing Data

Testing Data

Related Product Information for Egf recombinant protein

Human epidermal growth factor (EGF) is also known as HOMG4 and URG,and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk. plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR

Product Categories/Family for Egf recombinant protein

Cancer Research; Growth Factors; Cytokines & Growth Factors (A-Z); Proteins; Enzymes; Proteins & Enzymes (A-Z); Cytokines; Growth Factors & Hormones; EGFs; Cytokines & Growth Factors (A-Z); Human cell expressed recombinant proteins

NCBI and Uniprot Product Information

• NCBI GI #: 170172524
• NCBI GeneID: 13645
• NCBI Accession #: NP_034243.2
• NCBI GenBank Nucleotide #: NM_010113.4
• UniProt Accession #: P01132; Q569W5; Q6P9J2; E9QNX6
• Molecular Weight: 133,072 Da
• NCBI Official Full Name: pro-epidermal growth factor isoform 1
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: Egf
• NCBI Official Synonym Symbols: AI790464
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: Egf
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_MOUSE

Uniprot Description

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Ligand, receptor tyrosine kinase; Membrane protein, integral

Cellular Component: extracellular space; lysosomal membrane; plasma membrane; receptor complex

Molecular Function: epidermal growth factor receptor binding; growth factor activity; protein binding; protein-tyrosine kinase activity; Wnt receptor activity; Wnt-protein binding

Biological Process: activation of MAPK activity; activation of MAPKK activity; angiogenesis; branching morphogenesis of a tube; epidermal growth factor receptor signaling pathway; negative regulation of secretion; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor activity; positive regulation of fibroblast proliferation; positive regulation of granule cell precursor proliferation; positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of transcription, DNA-dependent; regulation of peptidyl-tyrosine phosphorylation; regulation of protein secretion; regulation of protein transport; STAT protein nuclear translocation; Wnt receptor signaling pathway through beta-catenin