Human MRGPRX2 (Mas-related G-protein coupled receptor member X2) ELISA Kit | MRGPRX2 elisa kit

Human MRGPRX2 (Mas-related G-protein coupled receptor member X2) ELISA Kit

Cat. Num. AAA7612225

• Gene Names: MRGPRX2; MGRG3; MRGX2
• Reactivity: Human
• Synonyms: MRGPRX2 (Mas-related G-protein coupled receptor member X2); Human MRGPRX2 (Mas-related G-protein coupled receptor member X2) ELISA Kit; Mas-related G-protein coupled receptor member X2; MRGPRX2; MRGX2; MRGPRX2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MRGPRX2. No significant cross-reactivity or interference between MRGPRX2 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MRGPRX2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16876451
• NCBI GeneID: 117194
• NCBI Accession #: NP_473371.1
• NCBI GenBank Nucleotide #: NM_054030.2
• UniProt Accession #: Q96LB1; Q4QXW4; Q4QXW7; Q4QXX0; Q4QXX2; Q4QXX3; Q4QXX4; Q4QXX6; Q4QXX7; B5B0C7
• Molecular Weight: 37,099 Da
• NCBI Official Full Name: mas-related G-protein coupled receptor member X2
• NCBI Official Synonym Full Names: MAS-related GPR, member X2
• NCBI Official Symbol: MRGPRX2
• NCBI Official Synonym Symbols: MGRG3; MRGX2
• NCBI Protein Information: mas-related G-protein coupled receptor member X2; G protein-coupled receptor MRGX2; Mas-related G protein-coupled receptor G3
• UniProt Protein Name: Mas-related G-protein coupled receptor member X2
• Protein Family: Mas-related G-protein coupled receptor
• UniProt Gene Name: MRGPRX2
• UniProt Synonym Gene Names: MRGX2
• UniProt Entry Name: MRGX2_HUMAN

Uniprot Description

MRGPRX2: Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin- stimulated cAMP levels, suggesting that this receptor is G(q)- coupled. Belongs to the G-protein coupled receptor 1 family. Mas subfamily.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide binding

Biological Process: positive regulation of cytokinesis; G-protein coupled receptor protein signaling pathway; sensory perception of pain; sleep