EGF active protein

EGF, 971-1023 aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA203083

• Gene Names: EGF; URG; HOMG4
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: EGF; 971-1023 aa; Human; E Coli (Bioactivity Validated); Epidermal growth factor; Pro-epidermal growth factor; urogastrone; epidermal growth factor (beta-urogastrone); EGF active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate-buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR
• Sequence Length: 1207

• Applicable Applications for EGF active protein: SDS-PAGE
• Antigen Species: Human
• Biological Activity: The ED50 for this effect is less or equal to 0.1 ng/ml. Measured in a cell proliferation assay using mouse Balb3T3 cell.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for EGF active protein

Recombinant human epidermal growth factor (EGF) is a 6.3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E Coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Categories/Family for EGF active protein

Hormones & Cytokines

References

Riese., et al (1998) Bioessays. 20: 41-48.; Cohen S., (1983) Cancer. 15: 1787-1791. ; Carpenter G., et al (1979) Annu. Rev. Biochem., 48: 193-216.;

NCBI and Uniprot Product Information

• NCBI GI #: 166362728
• NCBI GeneID: 1950
• NCBI Accession #: NP_001954
• NCBI GenBank Nucleotide #: NM_001963.5
• UniProt Accession #: P01133; Q52LZ6; B4DRK7; E7EVD2; E9PBF0
• Molecular Weight: 6.3kDa (54aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: pro-epidermal growth factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).