Testing Data
C5a active protein
C5a, human recombinant
Applications
SDS-Page
Purity
>=95%
Synonyms
C5a; human recombinant; C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor (C5a-R); (C5aR; ) (CD antigen CD88); C5AR1; C5AR; C5R1; Recombinant human C5a; C5a active protein
Host
E Coli
Purity/Purification
>=95%
Form/Format
Lyophilized with no additives
Appearance: Lyophilized protein
Appearance: Lyophilized protein
Applicable Applications for C5a active protein
SDS-PAGE; HPLC
Solubility/Reconstitution Instructions
Reconstitute to a concentration of 1.0 mg/ml in ice-cold H?O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70 degree C for future use. Frozen/thaw will result in some loss of activity.
Endotoxin Level
<0.1 ng/mug
Biological Activity
Human recombinant C5a is as active as that of serum derived C5a based on enzyme release assay of myeloperoxidase. The current lot of ED?? = 1.36 nmolar.
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for C5a active protein
Background: Human complement C5a is a 74-amino acid glycopeptide generated during complement activation from the alpha-chain of complement C5. C5a is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. The 74 amino acid, human C5a protein expressed in E. coli has an activity similar to that of serum derived C5a based on enzyme assay of myeloperoxidase release.
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Product Notes
The C5a (Catalog #AAA845172) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's C5a can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE; HPLC. Researchers should empirically determine the suitability of the C5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C5a, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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