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Western Blot (WB) (Western Blot detection against Immunogen (36.01kD).)

Mouse anti-Human TRIB2 Monoclonal Antibody | anti-TRIB2 antibody

TRIB2 (Tribbles Homolog 2, TRB2, TRB-2, C5FW, GS3955) (Biotin)

Gene Names
TRIB2; C5FW; TRB2; GS3955
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TRIB2; Monoclonal Antibody; TRIB2 (Tribbles Homolog 2; TRB2; TRB-2; C5FW; GS3955) (Biotin); anti-TRIB2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, lambda
Clone Number
1B1
Specificity
Recognizes human TRIB2.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-TRIB2 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa254-344 from human TRIB2 (NP_067675) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
FHDIEPSSLFSKIRRGQFNIPETLSPKAKCLIRSILRREPSERLTSQEILDHPWFSTDFSVSNSAYGAKEVSDQLVPDVNMEENLDPFFN*
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.01kD).)

Western Blot (WB) (Western Blot detection against Immunogen (36.01kD).)

Western Blot (WB)

(Western Blot analysis of TRIB2 expression in transfected 293T cell line by TRIB2 monoclonal antibody. Lane 1: TRIB2 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of TRIB2 expression in transfected 293T cell line by TRIB2 monoclonal antibody. Lane 1: TRIB2 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to TRIB2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to TRIB2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged TRIB2 is ~0.03ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged TRIB2 is ~0.03ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of TRIB2 over-expressed 293 cell line, cotransfected with TRIB2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIB2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Western Blot (WB) (Western blot analysis of TRIB2 over-expressed 293 cell line, cotransfected with TRIB2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIB2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Western Blot (WB)

(TRIB2 monoclonal antibody Western Blot analysis of TRIB2 expression in Jurkat.)

Western Blot (WB) (TRIB2 monoclonal antibody Western Blot analysis of TRIB2 expression in Jurkat.)
Product Categories/Family for anti-TRIB2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,801 Da
NCBI Official Full Name
tribbles homolog 2
NCBI Official Synonym Full Names
tribbles pseudokinase 2
NCBI Official Symbol
TRIB2
NCBI Official Synonym Symbols
C5FW; TRB2; GS3955
NCBI Protein Information
tribbles homolog 2
UniProt Protein Name
Tribbles homolog 2
Protein Family
UniProt Gene Name
TRIB2
UniProt Synonym Gene Names
TRB-2
UniProt Entry Name
TRIB2_HUMAN

NCBI Description

This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

TRB2: Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 2p24.3

Cellular Component: cytoplasm; cytoskeleton; nucleus

Molecular Function: mitogen-activated protein kinase kinase binding; nucleotide binding; protein kinase activity; protein kinase inhibitor activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity

Biological Process: negative regulation of fat cell differentiation; negative regulation of interleukin-10 biosynthetic process; negative regulation of protein kinase activity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of MAP kinase activity

Research Articles on TRIB2

Similar Products

Product Notes

The TRIB2 trib2 (Catalog #AAA6144915) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TRIB2 (Tribbles Homolog 2, TRB2, TRB-2, C5FW, GS3955) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIB2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 3ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TRIB2 trib2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIB2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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