Rabbit C5aR Polyclonal Antibody | anti-C5aR antibody

C5aR (S334) Polyclonal Antibody

Cat. Num. AAA3001229

• Gene Names: C5AR1; C5A; C5AR; C5R1; CD88
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: C5aR; Polyclonal Antibody; C5aR (S334) Polyclonal Antibody; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5a-R; CD88; C5AR1; C5AR; C5R1; anti-C5aR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CD88/C5aR (S334) polyclonal antibody detects endogenous levels of D88/C5aR protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 350

• Applicable Applications for anti-C5aR antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of C5aR (S334) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-C5aR antibody

C5a receptor (C5aR), also known as CD88, is a G protein-coupled integral membrane protein. CD88, which is expressed on neutrophils, monocytes, macrophages, hepatocytes and mast cells, as well as on various epithelial and endothelial cells, serves as a receptor for the inflammatory peptide C5a. Research studies suggest a role for CD88 in the inflammatory response. The binding of C5a to CD88 has been shown to elicit increased production of acute phase proteins in liver. In brain, an increased production of CD88 has been shown to be associated with inflammation. Research also indicates a role for C5a/C5aR in the pathogenesis of rheumatoid arthritis, as well as a heightened responsiveness of human bronchial epithelial cells (HBECs) to C5a upon exposure of these cells to cigarette smoke and other environmental irritants.

NCBI and Uniprot Product Information

• NCBI GI #: 4502509
• NCBI GeneID: 728
• NCBI Accession #: NP_001727.1
• NCBI GenBank Nucleotide #: NP_001727.1
• UniProt Accession #: P21730
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: C5a anaphylatoxin chemotactic receptor 1
• NCBI Official Synonym Full Names: complement C5a receptor 1
• NCBI Official Symbol: C5AR1
• NCBI Official Synonym Symbols: C5A; C5AR; C5R1; CD88
• NCBI Protein Information: C5a anaphylatoxin chemotactic receptor 1
• UniProt Protein Name: C5a anaphylatoxin chemotactic receptor
• UniProt Gene Name: C5AR1
• UniProt Synonym Gene Names: C5AR; C5R1; C5a-R; C5aR
• UniProt Entry Name: C5AR_HUMAN

Uniprot Description

C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: cell surface; basolateral plasma membrane; integral to plasma membrane; apical part of cell; cytoplasmic membrane-bound vesicle; plasma membrane

Molecular Function: complement component C5a receptor activity; complement component C5a binding; C5a anaphylatoxin receptor activity

Biological Process: neutrophil chemotaxis; organ regeneration; sensory perception of chemical stimulus; activation of MAPK activity; apoptosis; response to lipopolysaccharide; chemotaxis; cell proliferation in hindbrain; signal transduction; mRNA transcription from RNA polymerase II promoter; elevation of cytosolic calcium ion concentration; defense response to Gram-positive bacterium; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation