Testing Data
General Assay Diluent
General Assay Diluent
Synonyms
General Assay Diluent; General Assay Diluent diluent
Form/Format
Clear to light yellow liquid
pH
7.2-7.6
Specification
1X ready to use
Instructions
1. Dilute the standard curve, controls, and the samples as necessary. ICT off ers several formu-lations of sample diluents in which to prepare the samples.
2. Pipette 50-100 μL General Assay Diluent per well into every well of the plate.
3. Pipette 50-200 μL of each standard, control, and sample into the plate.
4. Run the assay according to the specific ELISA protocol.
5. Analyze the data. Because all of the wells, including the standards and controls, received the same volume of assay diluent, there is no need to account for this dilution when calculat-ing the results.
2. Pipette 50-100 μL General Assay Diluent per well into every well of the plate.
3. Pipette 50-200 μL of each standard, control, and sample into the plate.
4. Run the assay according to the specific ELISA protocol.
5. Analyze the data. Because all of the wells, including the standards and controls, received the same volume of assay diluent, there is no need to account for this dilution when calculat-ing the results.
Preparation and Storage
Store at 2-8°C. Store at room temperature for one week.
Testing Data
Testing Data
Related Product Information for General Assay Diluent diluent
General Assay Diluent is formulated for testing serum, plasma, urine, and cell cul-ture samples in all sandwich ELISA formats. Use of an assay diluent helps minimize matrix complexity diff er-ences between the sample (e.g., serum, etc.) and the diluent used to generate the standard curve of the ELISA. Large diff erences between the sample and the standard diluent matrices will result in under-recovery of the target analyte present in sample wells. Complex and concentrated protein environments (the matrix) present in serum or plasma samples will greatly reduce the antigen-binding eff iciency of the plate-adsorbed antibodies, resulting in a gross underestimation of the amount of target analyte present in the test samples. General Assay Diluent helps equalize the antibody-binding eff iciencies between the standard curve and the sample wells.
To use, simply add 50-100 μL to every well of the ELISA plate, including all wells designated for standards, controls, and samples. Then add the standards, controls, and samples to the plate. Mammalian protein ad-ditives included in the formulation serve to reduce non-specific interac-tions between the sample matrix proteins and the plate surface, thereby minimizing background noise. General Assay Diluent also inhibits com-plement and thrombin activity present in serum and plasma samples. Incorporation of an antimicrobial agent allows for room temperature bench-top use and extensive storage stability at 2-8°C. This assay diluent formulation ensures a more accurate and consistent ELISA performance, regardless of sample type.
To use, simply add 50-100 μL to every well of the ELISA plate, including all wells designated for standards, controls, and samples. Then add the standards, controls, and samples to the plate. Mammalian protein ad-ditives included in the formulation serve to reduce non-specific interac-tions between the sample matrix proteins and the plate surface, thereby minimizing background noise. General Assay Diluent also inhibits com-plement and thrombin activity present in serum and plasma samples. Incorporation of an antimicrobial agent allows for room temperature bench-top use and extensive storage stability at 2-8°C. This assay diluent formulation ensures a more accurate and consistent ELISA performance, regardless of sample type.
Product Categories/Family for General Assay Diluent diluent
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Product Notes
The General Assay Diluent (Catalog #AAA258385) is a Diluent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "General Assay Diluent, Diluent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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