Rabbit anti-Human, Mouse ME2 Polyclonal Antibody | anti-ME2 antibody

ME2, CT (ME2, NAD-dependent malic enzyme, mitochondrial, Malic enzyme 2) (APC)

Cat. Num. AAA6319860

• Gene Names: ME2; ODS1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ME2; Polyclonal Antibody; CT (ME2; NAD-dependent malic enzyme; mitochondrial; Malic enzyme 2) (APC); Malic enzyme 2; anti-ME2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ME2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ME2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 526-556 amino acids from the C-terminal region of human ME2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(ME2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ME2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(ME2 Antibody (C-term) western blot analysis in mouse NIH-3T3,Neuro-2a cell line lysates (35ug/lane).This demonstrates the ME2 antibody detected the ME2 protein (arrow).)

Related Product Information for anti-ME2 antibody

This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq].

Product Categories/Family for anti-ME2 antibody

Antibodies; Mitochondrial Proteins

References

MacDonald, M.J., et al. Arch. Biochem. Biophys. 488(2):100-104(2009). French, D., et al. Blood 113(19):4512-4520(2009). Escamilla, M. Pharmacogenomics 8(7):691-695(2007). Chou, W.Y., et al. Biochem. Biophys. Res. Commun. 357(1):133-138(2007). Lenzen, K.P., et al. Epilepsia 46(10):1637-1641(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4505145
• NCBI GeneID: 4200
• NCBI Accession #: NP_002387.1
• NCBI GenBank Nucleotide #: NM_002396.4
• UniProt Accession #: P23368; Q9BWL6; Q9BYG1; Q9H4B2; B2R8J2
• Molecular Weight: 53,586 Da
• NCBI Official Full Name: NAD-dependent malic enzyme, mitochondrial isoform 1
• NCBI Official Synonym Full Names: malic enzyme 2, NAD(+)-dependent, mitochondrial
• NCBI Official Symbol: ME2
• NCBI Official Synonym Symbols: ODS1
• NCBI Protein Information: NAD-dependent malic enzyme, mitochondrial
• UniProt Protein Name: NAD-dependent malic enzyme, mitochondrial
• UniProt Gene Name: ME2
• UniProt Synonym Gene Names: NAD-ME
• UniProt Entry Name: MAOM_HUMAN

NCBI Description

This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

ME2: a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]

Protein type: Oxidoreductase; EC 1.1.1.38; Mitochondrial; Carbohydrate Metabolism - pyruvate

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: intracellular membrane-bound organelle; mitochondrion; mitochondrial matrix

Molecular Function: malate dehydrogenase (decarboxylating) activity; electron carrier activity; metal ion binding; NAD binding; oxaloacetate decarboxylase activity

Biological Process: malate metabolic process

Disease: Opioid Dependence, Susceptibility To, 1; Epilepsy, Idiopathic Generalized