Typical Testing Data/Standard Curve (for reference only)
Mouse Diacylglycerol kinase alpha ELISA Kit | Dgka elisa kit
Mouse Diacylglycerol kinase alpha ELISA Kit
Gene Names
Dgka; 80kDa; Dagk1; AW146112
Reactivity
Mouse
Synonyms
Diacylglycerol kinase alpha; Mouse Diacylglycerol kinase alpha ELISA Kit; DAG kinase alpha; 80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGK-alpha; Dgka; Dagk1; 2.7.1.107; Dgka elisa kit
Reactivity
Mouse
Specificity
Recombinant and natural Mouse Diacylglycerol kinase alpha,DAG kinase alpha
Sequence Length
730
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
0.625-40 ng/mL
Sensitivity
<0.316 ng/mL
Intra-assay Precision
Intra-Assay CV: <=8.3%
Inter-assay Precision
Inter-Assay CV: <=10.1%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Dgka elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,762 Da
NCBI Official Full Name
diacylglycerol kinase alpha
NCBI Official Synonym Full Names
diacylglycerol kinase, alpha
NCBI Official Symbol
Dgka
NCBI Official Synonym Symbols
80kDa; Dagk1; AW146112
NCBI Protein Information
diacylglycerol kinase alpha
UniProt Protein Name
Diacylglycerol kinase alpha
Protein Family
UniProt Gene Name
Dgka
UniProt Synonym Gene Names
Dagk1; DAG kinase alpha; DGK-alpha
UniProt Entry Name
DGKA_MOUSE
Uniprot Description
DGKA: Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. Monomer. Lymphocytes and oligodendroglial cells. Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C. Belongs to the eukaryotic diacylglycerol kinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, lipid; Lipid Metabolism - glycerolipid; Motility/polarity/chemotaxis; Lipid Metabolism - glycerophospholipid; EC 2.7.1.107
Cellular Component: cytosol; membrane; plasma membrane
Molecular Function: ATP binding; calcium ion binding; diacylglycerol kinase activity; kinase activity; metal ion binding; nucleotide binding; phospholipid binding; transferase activity
Biological Process: phosphorylation; protein kinase C activation
Research Articles on Dgka
Similar Products
Product Notes
The Mouse Dgka dgka (Catalog #AAA2885080) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2885080 ELISA Kit recognizes Mouse Dgka. It is sometimes possible for the material contained within the vial of "Diacylglycerol kinase alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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